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The development of the myocardium and endocardium in mouse embryos

Fusion of two heart tubes?

Anatomy and Embryology volume 185, pages 461–473 (1992)Cite this article

Summary

The formation of the single heart tube by hypothetical fusion of two separately developed heart tubes is re-investigated, because this intricate process is ambiguously and often incompletely described. To gain a better insight into this problem ten mouse embryos ranging from 7.5 to 8.5 days of development (presomite to 6 somites) were serially sectioned (1 μm) and reconstructed graphically. Twenty mouse embryos of comparative ages, were studied by scanning electron microscopy. Two large embryonic mesodermal compartments, derived from the primitive streak, extend rostrally on either side of the embryonic axis, and meet in front of the buccopharyngeal membrane. In each compartment a coelomic cavity develops, splitting the mesoderm into a splanchnic and somatic layer. The splanchnic mesoderm differentiates into a layer of cuboidal splanchnic mesothelial cells (promyocardium) and a subjacent plexus of elongated endothelial cells (proendocardium). Before the 1-somite stage the left and right splanchnic mesoderm are separated in front of the buccopharyngeal membrane by a thickening of the yolk sac endoderm. The splanchnic mesoderm then fuses, forming a single horseshoe-shaped heart primordium consisting of a promyocardial layer and a subjacent vascular plexus. Until the 2-somite stage both coelomic cavities remain separated by a bilayer of squamous somatic mesothelial cells (‘mesocardium’). The plexus of endothelial cells that forms the proendocardium, also seems to be the source of the lining of the vitelline veins, the pharyngeal arch arteries and the dorsal aortae. The relatively close adherence of endoderm to the medial part of the horseshoeshaped heart primordium, combined with a bilateral accumulation of cardiac jelly, is suggestive of a double heart tube. However, promyocardium and proendocardium are both translocated as one horseshoe-shaped layer, thus fusion of the left and right parts of the heart primordium does not occur.

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References

  • Bredman JJ, Weijs WA, Moorman AFM (1990) Expression of ‘cardiac-specific’ myosin heavy chain in rabbit cranial muscles. In: Maréchal G, Carraro U (eds) Muscle and motility, vol 2, Proc 19 Eur Conf in Brussels. Intercept, Andover, Hampshire, UK, pp 329–335

    Google Scholar 

  • Bredman JJ, Wessels A, Weijs WA, Korfage JAM, Soffers CAS, Moorman AFM (1991) Demonstration of ‘cardiac-specific’ myosin heavy chain in masticatory muscles of human and rabbit. Histochem J (in press)

  • Coffin JD, Poole TJ (1988) Embryonic vascular development: immunohistochemical identification of the origin and subsequent morphogenesis of the major vessel primordia in quail embryos. Development 102:735–748

    Google Scholar 

  • Davis CL (1927) Development of the human heart from its first appearance to the stage found in embryos of twenty paired somites. Carnegie Inst Contr Embryol 19:245–284

    Google Scholar 

  • DeHaan RL (1963) Migration patterns of the precardiac mesoderm in the early chick embryo. Exp Cell Res 29:544–560

    Google Scholar 

  • DeJong F, Geerts WJC, Lamers WH, Los JA, Moorman AFM (1990) Isomyosin expression pattern during formation of the tubular chicken heart: a three-dimensional immunohistochemical analysis. Anat Rec 226:213–227

    Google Scholar 

  • DeRuiter MC, Gittenberger-de Groot AC, Rammos S, Poelmann RE (1989) The special status of the pulmonary arch artery in the branchial arch system of the rat. Anat Embryol 179:319–325

    Google Scholar 

  • Drake CJ, Davis LA, Walters L, Little CD (1990) Avian vasculogenesis and the distribution of collagens I, IV, Laminin, and fibronectin in the heart primordia. J Exp Zool 255:309–322

    Google Scholar 

  • Goedbloed JF, Smits-van Prooije AE (1986) A quantitative analysis of the temporal pattern of somite formation in mouse and rat. A simple and accurate method for age determination. Acta Anat 125:76–82

    Google Scholar 

  • Hamilton WJ, Boyd JD, Mossman HW (1972) Human embryology. Prenatal development of form and function. 4th edn. Heffer, Cambridge. Williams & Wilkins, Baltimore

    Google Scholar 

  • Holmdahl DE (1941) Wann und wie entsteht das Blutgefäßsystem beim Menschen? Z Mikrosk Anat Forsch 49:130–174

    Article  Google Scholar 

  • Hudson L, Hay FC (1976). Practical immunology. Blackwell, London

    Google Scholar 

  • Hurle JM, Ojeda JL (1977) Cardiac jelly arrangement during formation of the tubular heart of the chick embryo. Acta Anat 98:444–455

    Google Scholar 

  • Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27:137A

    Google Scholar 

  • Kaufman MH, Navaratnam V (1981) Early differentiation of the heart in mouse embryos. J Anat 133:235–246

    Google Scholar 

  • Kuhn HJ, Liebherr G (1987) The early development of the heart of Tupaia belangeri, with reference to other mammals. Anat Embryol 176:53–63

    Google Scholar 

  • Langemeijer RATM (1987) The coelom. A descriptive morphological research of the genesis and the development of the coelom and the coelomic wall. Thesis, Amsterdam

  • Manasek FJ (1968) Embryonic development of the heart. 1. A light and electron microscopic study of myocardial development in the early chick embryo. J Morphol 125:329–366

    Google Scholar 

  • M'Intyre D (1926) The development of the vascular system in the human embryo prior to the establishment of the heart. Trans R Soc Edin 55:77–113

    Google Scholar 

  • Moore KL (1988) The developing human. Clinically oriented embryology. 4th edn. Saunders, Philadelphia

    Google Scholar 

  • Noden DM (1989) Embryonic origins and assembly of blood vessels. Am Rev Respir Dis 140:1097–1103

    Google Scholar 

  • Noden DM (1990) Origins and patterning of avian outflow tract endocardial tissues and cushion mesenchyme. Anat Rec 226:72A-73A

    Google Scholar 

  • Noden DM (1991) Development of craniofacial blood vessels. In: Feinberg RN, Sherer GK, Auerbach R (eds) The development of the vascular system. Issues Biomed. Karger, Basel 14:1–24

  • O'Rahilly R, Müller F (1987) Developmental stages in human embryos. Carnegie Inst Washington. Publication 637, pp 93–104, Washington. ISBN 0-87279-666-3

  • Orts Llorca F (1934) Beschreibung eines menschlichen Embryo mit 4 Urwirbelpaaren. Z Anat Entwicklungsgesch 103:765–792

    Google Scholar 

  • Patten BM (1968) Human embryology. 3rd edn. McGraw-Hill, New York

    Google Scholar 

  • Patten BM (1971) Early embryology of the chick. 5th edition. McGraw-Hill, New York

    Google Scholar 

  • Poole TJ, Coffin JD (1988) Developmental angiogenesis: quail embryonic vasculature. Scanning Microsc 2:443–448

    Google Scholar 

  • Robinson A (1902) The early stages of the development of the pericardium. J Anat Physiol 37:1–16

    Google Scholar 

  • Romanoff AL (1960) The avian embryo. Structural and functional development. Macmillan, New York

    Google Scholar 

  • Rosenquist GC, DeHaan RL (1966) Migration of precardiac cells in the chick embryo: a radioautographic study. Carnegie Inst Contr Embryol 38(263): 111–121

    Google Scholar 

  • Rugh R (1968) The mouse. Its reproduction and development. Burgess, Minneapolis

    Google Scholar 

  • Stalsberg H, DeHaan RL (1969) The precardiac areas and formation of the tubular heart in the chick embryo. Dev Biol 19:128–159

    Google Scholar 

  • Steding G, Seidl W, Kluth D, Schulze M (1980) Die Entstehung des Endocard. Untersuchungen an Hühnerembryonen. Verh Anat Ges 74:365–367

    Google Scholar 

  • Sternberg H (1927) Beschreibung eines menschlichen Embryos mit vier Ursegmentpaaren, nebst Bemerkungen über die Anlage und früheste Entwicklung einiger Organe beim Menschen. Z Anat Entwickl.Ges 82:142–240

    Google Scholar 

  • Tam PPL, Beddington RSP (1986) The metameric organization of the presomitic mesoderm and somite specification in the mouse embryo. In: Bellairs R, Ede DA, Lash JW (eds) Somites in developing embryos. NATO ASI Series Serie A: Sciences 118:17–36

  • Theiler K (1972) The house mouse. Development and normal stages from fertilization to 4 weeks of age. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Tinkelenberg J (1979) A grafic reconstruction, microanatomy with a pencil. J Audiovis Media Med 2:102–106

    Google Scholar 

  • VanMierop LHS (1978) Embryology. In: Netter FH (prep), Yonkman FF (ed) The Ciba collection of medical illustrations, vol 5. Ciba, New York, pp 112–132

    Google Scholar 

  • Virágh Sz, Szabo E, Challice CE (1989) Formation of the primitive myo- and endocardial tubes in the chicken embryo. J Mol Cell Cardial 21:123–137

    Google Scholar 

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Affiliations

  1. Department of Anatomy and Embryology, University of Leiden, P.O. Box 9602, NL-2300, RC Leiden, The Netherlands

    M. C. DeRuiter, R. E. Poelmann, I. VanderPlas-de Vries, M. M. T. Mentink & A. C. Gittenberger-de Groot

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  1. M. C. DeRuiter
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  2. R. E. Poelmann
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  3. I. VanderPlas-de Vries
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  4. M. M. T. Mentink
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  5. A. C. Gittenberger-de Groot
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DeRuiter, M.C., Poelmann, R.E., VanderPlas-de Vries, I. et al. The development of the myocardium and endocardium in mouse embryos. Anat Embryol 185, 461–473 (1992). https://doi.org/10.1007/BF00174084

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Key words

  • Coelom
  • Endocardium
  • Mesoderm
  • Myocardium
  • Vasculogenesis