Summary
Chromosomal DNA patterns using the transverse alternating field electrophoresis technique and mitochondrial DNA restriction profiles have been achieved for 22 enological strains of Saccharomyces cerevisiae. Both methods have evidenced a marked polymorphism of these strains. Twenty different karyotypes and 17 mitochondrial DNA banding patterns have been observed. Only three strains originating from the same vineyard could not be differentiated by either of the two methods. The polymorphism observed at the chromosomal and mitochondrial levels makes the techniques investigated powerful tools for identification and control of industrial strains.
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References
Aigle M, Erbs D, Moll M (1984) Some molecular structures in the genome of larger brewing yeast. Am Soc Brew Chem 42:1–7
Blondin B, Vezinhet F (1988) Identification de souches de levures oenologiques par leurs caryotypes obtenus en électrophorèse en champ pulsé. Rev Fr Oenol 28:7–11
Carle GF, Olson MV (1985) An electrophoretic karyotype for yeast. Proc Natl Acad Sci USA 82:3756–3760
Casey GP, Xiao W, Rank GH (1988) Application of pulsed field chromosome electrophoresis in the study of chromosome XIII and electrophoretic karyotype of industrial strains of Saccharomyces yeasts. J Inst Brew 94:239–243
De Jonge P, De Jonge FCM, Meijers R, Steensma HY, Scheffers WA (1986) Orthogonal-field-alternation gel electrophoresis banding patterns of DNA from yeasts. Yeast 2:193–204
Dubourdieu D, Sokol A, Zucca P, Thalouarn P, Dattee A, Aigle M (1987) Identification des souches de levures isolées de vins par l'analyse de leur ADN mitochondrial. Connaiss Vigne Vin 21:267–278
Dujon B (1981) Mitochondrial genetics and function. In: Strathern JN, Jones EW, Broache JR (eds) The molecular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 505–605
Dujon B, Blanc H (1980) Yeast mitochondria minilysates and their use to screen a collection of hypersuppressive p − mutants. In: Kroon AM, Saccone C (eds) The organization and expression of the mitochondrial genome. Elsevier, Amsterdam, pp 33–37
Hallet JN, Craneguy B, Zucca J, Poulard A (1988) Caracterisation de differentes souches industrielles de levures oenologiques par les profils de restriction de leur ADN mitochondrial. Prog Agric Vitic 105:328–333
Hudspeth M, Shumard D, Tatti K (1980) Rapid purification of yeast mitochondrial DNA in high yield. Biochem Biophys Acta 610:221–228
Johnston JR, Mortimer RK (1986) Electrophoretic karyotyping of laboratory and commercial strains of Saccharomyces and other yeasts. Int J Syst Bacteriol 36:569–572
Johnston JR, contopoulou CR, Mortimer RK (1988) Karyotyping of yeast strains of several genera by field inversion gel electrophoresis. Yeast 4:191–198
Lee SY, Knudsen FB (1985) Differentiation of brewery yeast strains by restriction endonuclease analysis of their mitochondrial DNA. J Inst Brew 91:169–173
Loiseau G (1986) Obtention d'un marquage mitochondrial de levures oenologiques sélectionnées en champagne. Application au contróle microbiologique en oenologie. Thesis, Université de Sciences et Techniques du Languedoc, Montpellier
Pedersen MB (1986a) DNA sequence polymorphisms in the genus Saccharomyces III. Restriction endonulease fragment patterns of chromosomal regions in brewing and other yeast strains. Carlsberg Res Commun 51:163–183
Pedersen MB (1986b) DNA sequence polymorphisms in the genus Saccharomyces IV. Homologous chromosomes III of Saccharomyces bayanus, S. Carlsbergensis and S. uvarum. Carlsberg Res Commun 51:185–202
Petering J, Langridge P, Henschke P (1988) Fingerprinting wine yeasts. Aust N Z Wine Ind J 3:48–52
Sor F, Fukuhara H (1989) Analysis of chromosomal DNA patterns of the genus Kluyveromyces. Yeast 5:1–10
Vezinhet F (1985) Le marquage génétique de souches de levures oenologiques. Rev Fr Oenol 25:47–51
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Vezinhet, F., Blondin, B. & Hallet, JN. Chromosomal DNA patterns and mitochondrial DNA polymorphism as tools for identification of enological strains of Saccharomyces cerevisiae . Appl Microbiol Biotechnol 32, 568–571 (1990). https://doi.org/10.1007/BF00173729
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DOI: https://doi.org/10.1007/BF00173729