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Lactic acid production from lactose in batch culture: analysis of the data with the help of a mathematical model; relevance for nitrogen source and preculture assessment

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Abstract

Batch fermentation performances are usually optimized on the basis of an overall criterion, the mean volumetric productivity. For lack of more suitable criteria, a great number of experiments have to be carried out under various conditions, in order to identify the factors acting on product formation rate. With the help of a mathematical model, every batch fermentation is quantitatively described by a set of parameters, so the reason of every improvement observed for the fermentation productivity is easy to recognize. Therefore, such a model appears to be an invaluable tool for finding quickly and at lower expense the optimal conditions. Nitrogen supplementation and inoculum preparation for lactobacilli growing on whey and whey permeate have been assessed with the help of a new mathematical model.

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References

  • Adler-Nissen J (1984) Control of the proteolytic reaction and of the level of bitterness in protein hydrolysis processes. J Chem Technol Biotechnol 34B:215–222

    Google Scholar 

  • Aeschlimann A, Stockar U von (1989) The production of lactic acid from whey permeate by Lactobacillus helveticus. Biotechnol Lett 11:195–200

    Google Scholar 

  • Aeschlimann A, Stockar U von (1990) The effect of yeast extract supplementation on the production of lactic acid from whey permeate by Lactobacillus helveticus. Appl Microbiol Biotechnol 32:398–402

    Google Scholar 

  • Amrane A (1991) Optimisation de la productivité d'un fermenteur effectuant la conversion en acide lactique de perméat de lactosérum supplémenté. PhD dissertation, Rennes I University, France

    Google Scholar 

  • Ayroulet-Martin M, Fournaud J (1979) Application de la méthode de Ling au dosage de l'acide lactique dans les viandes. Ann Technol Agric (Paris) 28:333–343

    Google Scholar 

  • Desmaison A-M, Breton MJ-C, Tixier M, Chatelut MJ (1970) Dosage colorimétrique du lactose dans le lait. Bull Soc Pharm Bord 109:118–131

    Google Scholar 

  • Desmazeaud M (1983) L'état des connaissances en matiére de nutrition des bactéries lactiques. Le Lait 63:267–316

    Google Scholar 

  • Herbert D, Phipps PJ, Strange RE (1971) Chemical analysis of microbial cells. Methods Enzymol 5B:265–308

    Google Scholar 

  • Jacobsen CF, Leonis J, Linderstr∅m-Lang K, Ottesen M (1957) The pH-stat and its use in biochemistry. Methods Biochem Anal 4:171–210

    Google Scholar 

  • Leh MB (1987) The effect of whey protein hydrolyzates on the lactic acid fermentation. PhD dissertation, Lehigh University, Bethlehem, Pa.

    Google Scholar 

  • Leh MB, Charles M (1989a) Lactic acid production by batch fermentation of whey permeate: a mathematical model. J Ind Microbiol 4:65–70

    Google Scholar 

  • Leh MB, Charles M (1989b) The effect of whey protein hydrolyzates on the lactic acid fermentation. J Ind Microbiol 4:71–75

    Google Scholar 

  • Leh MB, Charles M (1989c) The effect of whey protein hydrolyzate average molecular weight on the lactic acid fermentation. J Ind Microbiol 4:77–80

    Google Scholar 

  • Luedeking R, Piret EL (1959) A kinetic study of the lactic acid fermentation. Batch process at controlled pH. J Biochem Microbiol Technol Eng 1:393–412

    Google Scholar 

  • Rodier J (1975) L'analyse de l'eau, vol 1. Dunod, Paris, pp 123–127

    Google Scholar 

  • Roy D, Goulet J, LeDuy A (1986) Batch fermentation of whey ultrafiltrate by Lactobacillus helveticus for lacic acid production. Appl Microbiol Biotechnol 24:206–213

    Google Scholar 

  • Roy D, LeDuy A, Goulet J (1987) Kinetics of growth and lactic acid production from whey permeate by Lactobacillus helveticus. Can J Chem Eng 65:597–603

    Google Scholar 

  • Satake K, Okuyama T, Ohashi M, Shinoda T (1960) The spectrophotometric determination of amine, amino acid and peptide with 2,4,6-trinitrobenzene 1-sulfonic acid. J Biochem 47:654–660

    Google Scholar 

  • Vahvaselkä MI, Linko P (1987) Lactic acid fermentation in milk ultrafiltrate by Lactobacillus helveticus. In: Neijssel OM, van der Meer RR, Luyben KChAM (eds) Proceedings of the 4th European Congress on Biotechnology, vol 3. Elsevier Science Publishers B. V., Amsterdam, pp 317–320

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire des procédés de séparation, I.U.T., Départment Chimie, Université de Rennes I, B. P. 1144, 35014, Rennes Cedex, France

    A. Amrane & Y. Prigent

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  1. A. Amrane
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  2. Y. Prigent
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Correspondence to: Y. Prigent

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Amrane, A., Prigent, Y. Lactic acid production from lactose in batch culture: analysis of the data with the help of a mathematical model; relevance for nitrogen source and preculture assessment. Appl Microbiol Biotechnol 40, 644–649 (1994). https://doi.org/10.1007/BF00173322

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  • DOI: https://doi.org/10.1007/BF00173322

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