Abstract
Infection causes remarkable changes in extracellular fluid volume, blood flow and oxygen consumption in the region of the lesion. To determine the sequence and magnitude of these changes, we performed serial scintigraphic measurements in 10 rabbits with experimental Escherichia coli abscesses. Positron emission tomography with C15O2, 15O2 and 11CO was used to measure regional blood flow, oxygen extraction (OEF) and blood volume; extracellular fluid volume was evaluated by single photon scintigraphy with indium-111 immunoglobulin G (IgG). Images were recorded following tracer administration at 1 and 7–10 days after infection. At the first imaging time, blood flow to infected muscle had increased by 40% compared with control sites (7.4±0.6 to 10.8 ± 3.8 ml/min · 100 g), OEF had decreased from 55%±34% to 45%±14%, and the infected-tocontralateral (I/C) ratio of IgG had increased to 3.34±1.85. At the later imaging time, flow had increased by almost threefold compared with day 1 (29.4±9.8 ml/min · 100 g), OEF had decreased to 29%±14%, and the I/C ratio for IgG had remained constant. Although OEF fell,oxygendelivery (OEF × flow) increased from 4.07 ml/min (control value) to 4.86 ml/min on day 1 and 8.64 ml/min on days 7–9. The infected-to-contralateral (IC) ratio of 1502/C15O2 was 0.74±0.15 on day 1 and 0.77±0.10 at 7–9 days. These studies indicate that expansion of the extracellular fluid volume increases early in the evolution of the infection and exceeds changes in regional perfusion and oxygen delivery.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bergstrom M, Litton J, Ericksson L, Bohm C, Blomquist G (1982) Determination of object contour from projections for attenuation correction in cranial positron emission tomography. J Comput Assist Tomogr 6:365–372
Clark JC, Buckingham PG (1975) In: Short lived radioactive gases for clinical use. Butterworth, London, pp 122–170, 215–260
Cotran RS, Kumar V, Robbins SL (1989) Inflammation and repair. In: Robbins pathologic basis of disease. 4th edn. WB Saunders, Philadelphia, pp 39–71
Fischman AJ, Rubin RH, Khaw BA, Callahan RJ, Wilkinson R, Keech F, Nedelman M, Dragotakes S, Kramer PB, LaMuraglia GM, Lind S, Strauss HW (1988) Detection of acute inflammation with 11In-labeled nonspecific polyclonal IgG. Semin Nucl Med 18:335–344
Fischman AJ, Rubin RH, White JA, Locke E, Wilkinson RA, Nedelman M, Callahan RJ, Khaw BA, Strauss HW (1990) Localization of Fc and Fab fragments of non-specific polyclonal IgG at focal sites of infection. J Nucl Med 31:1199–1205
Frackowiak RSJ, Lenzi GL, Jones T, Heather JD(1980) Quantitative measurement of regional cerebral blood flow and oxygen metabolism in man using 15O and positron emission tomography: theory, procedure and normal values. J Comput Assist Tomogr 4:727–736
Hay JB, Johnston MG, Hobbs BB, Movat HZ (1975) The use of radioactive microspheres to quantitate hyperemia in dermal inflammatory sites. Proc Soc Exp Biol Med 150:641–644
Jasin HE (1988) Oxidative crosslinking of immune complexes by human polymorphonuclear leukocytes. J Clin Invest 81:6–15
Juweid M, Fischman AJ, Rubin RH, Strauss HW(1991) In-111 labeled proteins in the extravascular compartment of normal muscle. Accumulation of immunoglobulin G at focal sites of infection. (Abstr) J Nucl Med 1057
Karieto AL, Brownell GL, Schluederberg J, Elmaleh DR (1983) Regional blood flow measurement in rabbit soft tissue tumors with positron imaging using C15O2 steady state method and labeled microspheres. J Nucl Med 24:1135–1142
Kopaniak MM, Issekutz AC, Burrowes CE, Movat HZ (1980a) The quantitation of hemorrhage in the microcirculation in inflammatory lesions and related phenomena. Proc Soc Exp Biol Med 163:126–131
Kopaniak MM, Issekutz AC, Movat HZ (1980b) Kinetics of acute inflammatory induced by E. coli in rabbits. Am J Pathol 98:485–498
Lammertsma AA, Jones T (1983) The correction for the presence of intravascular oxygen-15 in the steady-state technique for measuring regional oxygen extraction ratio in the brain: 1. Description of the method. J Cereb Blood Flow Metabol 3:416–424
Litton J, Bergstrom M, Eriksson L, Bohm C, Blomquist G (1984) Performance study of the PC-384 PET camera for the brain. J Comput Assist Tomogr 8:74–87
Morrell EM, Thompkins RG, Fischman AJ, Strauss HW, Rubin RH, Wilkinson RA, Yarmush ML (1989) An autoradiographic method for quantitation of radiolabeled proteins in tissue using indium-111. J Nucl Med 30:1538–1545
Rubin RH, Fischman AJ, Callahan RJ, Khaw BA, Keech F, Ahmad M, Wilkinson RA, Strauss HW (1989) 111In-labeled non-specific immunoglobulin scanning in the detection of focal infection. N Engl J Med 321:935–940
Senda M, Buxton RB, Alpert N, Correia J, Mackay BC, Weise SB, Ackerman RH (1988) The 150 steady-state model: correction for variation in arterial concentration. J Cereb Blood Flow Metab 8:681–690
Tunkel AR, Wispelwey B, Scheld WM (1990) Pathogenesis and pathophysiology of meningitis. Infect Dis Clin North Am 4:555–581
Udaka K, Takeuchi Y, Movat HZ (1970) Simple method for quantitation of enhanced vascular permeability. Proc Soc Exp Biol Med 133:1384–1387
Wilkinson RA, Fischman AJ, Rubin RH, Strauss HW (1989) Monitoring response to antimicrobial therapy with 111In-labeled polyclonal IgG. (Abstr) J Nucl Med 30:890
Wyler F (1974) Effect of general anesthesia on distribution of cardiac output and organ blood flow in the rabbit: halothane and chloralose-urethane. J Surg Res 17:381–386
Wyler F, Weisser K (1972) Effect of halothane anesthesia on distribution of cardiac output and organ blood flow in the rabbit. Br J Anaesth 44:551–555
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Senda, M., Fischman, A.J., Weise, S. et al. Regional perfusion, oxygen metabolism, blood volume and immunoglobulin G accumulation at focal sites of infection in rabbits. Eur J Nucl Med 19, 166–172 (1992). https://doi.org/10.1007/BF00173276
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00173276