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Determination of in-vivo cytoplasmic orthophosphate concentration in yeast

Biotechnology Techniques volume 10, pages 297–302 (1996)Cite this article

Summary

To investigate in-vivo concentrations of cytoplasmic phosphate, especially during dynamic conditions, a method has been developed that enables reproducible determination of cytoplasmic phosphate from 5 μM up to 30 μM. The method involves fast sampling, spontaneous inactivation of cell metabolism and differential extraction procedure to gain porosity of the outer cell membrane exclusively. To determine very low cytoplasmic phosphate concentrations, an enzymatic assay was linked to a sensitive spectrophotometric cycling method to increase the detection limit.

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Author notes

  1. Uwe Theobald

    Present address: Universität Tübingen, LS Mikrobiologie/Biotechnologie, Auf der Morgenstelle 28, D-72076, Tübingen, Germany

Authors and Affiliations

  1. Institut für Bioverfahrenstechnik, Universität Stuttgart, Allmandring 31, D-70569, Stuttgart, Germany

    Uwe Theobald, Jochen Mohns & Manfred Rizzi

Authors
  1. Uwe Theobald
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  2. Jochen Mohns
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  3. Manfred Rizzi
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Theobald, U., Mohns, J. & Rizzi, M. Determination of in-vivo cytoplasmic orthophosphate concentration in yeast. Biotechnol Tech 10, 297–302 (1996). https://doi.org/10.1007/BF00173242

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  • DOI: https://doi.org/10.1007/BF00173242

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