Skip to main content
SpringerLink
Log in

Neuropeptide Y potentiates via Y2-receptors the inhibitory effect of noradrenaline in rat locus coeruleus neurones

  • Short Communications
  • Published:
Naunyn-Schmiedeberg's Archives of Pharmacology Aims and scope Submit manuscript

Summary

Intracellular recordings were carried out in a pontine slice preparation of the rat brain containing the locus coeruleus (LC). Pressure application of noradrenaline with various pulse durations inhibited the spontaneous frequency of action potentials and hyperpolarized the membrane. Neuropeptide Y (NPY), its C-terminal fragment NPY(16–36) and peptide YY (PYY), at a concentration of 0.1 µmol/l all, potentiated the effect of noradrenaline, while [Leu31, Pro34]NPY (0.1 µmol/l) was inactive. These results are compatible with the presence of Y2-type NPY-receptors at the cell somata of LC neurones.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Aghajanian GK, VanderMaelen CP (1982) α2-Adrenoceptor-mediated hyperpolarization of locus coeruleus neurones: intracellular studies in vivo. Science 215:1394–1396

    Google Scholar 

  • Aghajanian GK, Wang YY (1987) Common α2- and opiate effector mechanisms in the locus coeruleus: intracellular studies in brain slices. Neuropharmacology 26:793–799

    Google Scholar 

  • Aicher SA, Springston M, Berger SB, Reis DJ, Wahlestedt C (1991) Receptor-selective analogs demonstrate NPY/PYY receptor heterogeneity in rat brain. Neurosci Lett 130:32–36

    Google Scholar 

  • Colmers WF, Klapstein GJ, Fournier A, St-Pierre S and Treherne KA (1991) Presynaptic inhibition by neuropeptide Y in rat hippocampal slice in vitro is mediated by a Y2 receptor. Br J Pharmacol 102:41–44

    Google Scholar 

  • Dahlstrom A, Fuxe K (1964) Evidence for the existence of monoamine-containing neurons in the central nervous system. I. Demonstration of monoamines in the cell bodies of brainstem neurons. Acta Physiol Scand 232:1–55

    Google Scholar 

  • Egan TM, Henderson G, North RA, Williams JT (1983) Noradrenaline-mediated synaptic inhibition in rat locus coeruleus neurones. J Physiol 345:477–488

    Google Scholar 

  • Finta EP, Regenold JT, Illes P (1992) Depression by neuropeptide Y of noradrenergic inhibitory postsynaptic potentials of locus coeruleus neurones. Naunyn-Schmiedeberg's Arch Pharmacol 346:472–474

    Google Scholar 

  • Holets VR, Hökfelt T, Rökaeus A, Terenius L, Goldstein M (1988) Locus coeruleus neurons in the rat containing neuropeptide Y, tyrosine hydroxylase or galanin and their efferent projections to the spinal cord, cerebral cortex and hypothalamus. Neuroscience 24:893–906

    Google Scholar 

  • Illes P, Regenold JT (1990) Interaction between neuropeptide Y and noradrenaline on central catecholamine neurons. Nature 344:62–63

    Google Scholar 

  • Illes P, Weber HD, Neuburger J, Bucher B, Regenold JT, Nörenberg W (1990) Receptor interactions at noradrenergic neurones. Ann NY Acad Sci 604:197–210

    Google Scholar 

  • Kombian SB, Colmers WIT (1992) Neuropeptide Y selectively inhibits slow synaptic potentials in rat dorsal raphe nucleus in vitro by a presynaptic action. J Neurosci 12:1086–1093

    Google Scholar 

  • Martire M, Pistritto G, Mores N, Agnati LF, Fuxe K (1993) Region-specific inhibition of potassium-evoked [3H]noradrenaline release from rat brain synaptosomes by neuropeptide Y(13–36). Involvement of NPY receptors of the Y2 type. Eur J Pharmacol 230:231–234

    Google Scholar 

  • Michel MC (1991) Receptors for neuropeptide Y: multiple subtypes and multiple second messengers. Trends Pharmacol Sci 12:389–394

    Google Scholar 

  • Tatemoto K, Carlquist M, Mutt V (1982) Neuropeptide Y — a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature 296:659–660

    Google Scholar 

  • Wahlestedt C, Grundemar L, Hakanson R, Heilig M, Shen GY, Zukowska-Grojec Z, Reis DJ (1990) Neuropeptide Y receptor subtypes, Y1 and Y2. Ann NY Acad Sci 611:7–26

    Google Scholar 

  • Williams JT and North RA (1984) Opiate-receptor interactions on single locus coeruleus neurones. Mol Pharmacol 26:489–497

    Google Scholar 

  • Williams JT, Henderson G, North RA (1985) Characterization of α2-adrenoceptors which increase potassium conductance in rat locus coeruleus neurones. Neuroscience 14:95–101

    Google Scholar 

  • Yokoo H, Schlesinger DH, Goldstein M (1987) The effect of neuropeptide Y (NPY) on stimulation-evoked release of [3H]norepinephrine (NE) from rat hypothalamic and cerebral cortical slices. Eur J Pharmacol 143:283–286

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology, University of Freiburg, Hermann-Herder-Strasse 5, D-79104, Freiburg, Germany

    P. Illes, E. P. Finta & K. Nieber

Authors
  1. P. Illes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. P. Finta
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Nieber
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Correspondence to: P. Illes at the above address

Rights and permissions

Reprints and permissions

About this article

Cite this article

Illes, P., Finta, E.P. & Nieber, K. Neuropeptide Y potentiates via Y2-receptors the inhibitory effect of noradrenaline in rat locus coeruleus neurones. Naunyn-Schmiedeberg's Arch Pharmacol 348, 546–548 (1993). https://doi.org/10.1007/BF00173217

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00173217

Key words

Search

Navigation