Abstract
In order to clarify whether adrenoceptors in the guinea-pig distal colon are under sympathetic control, we assessed possible variations in the sensitivity to adrenoceptor agonists after blockade of neuronal catecholamine uptake mechanisms by desipramine (DMI). First, experiments were carried out to investigate the effects of DMI added in the organ bath on propulsion velocity, endogenous and [3H] prelabelled acetylcholine overflow, electrically evoked noradrenaline overflow and longitudinal smooth muscle tone. Secondly, we studied the effects of adrenoceptor agonists on the above parameters in untreated animals and in animals chronically treated with DMI.
DMI added in the organ bath at concentrations equal to or higher than 30 nM inhibited all the parameters under study. Thus, when evaluating the effect of DMI on concentration-response curves to adrenoceptor agonists, concentrations which were per se inactive were used. DMI added in the organ bath at concentrations up to 30 nM potentiated the inhibitory effects of exogenous noradrenaline on propulsion velocity and acetylcholine overflow, but it did not affect the concentration-response curve to exogenous noradrenaline on longitudinal smooth muscle tone. Furthermore, 30 nM DMI inhibited propulsion velocity during sympathetic nerve stimulation. In preparations obtained from animals chronically treated with DMI, no significant change of propulsion velocity, endogenous and [3H] prelabelled acetylcholine overflow was found with respect to untreated animals. Nevertheless, in such preparations subsensitivity to isoprenaline (acting mainly on muscular β-adrenoceptors) and clonidine (acting on neuronal α2-adrenoceptors) and supersensitivity to phenylephrine were observed. Electrically evoked noradrenaline overflow was enhanced, in a frequency-dependent way, by yohimbine and inhibited by clonidine.
We conclude that in the guinea-pig colon: 1) α- and β-adrenoceptors are under tonic neuronal control, as indicated by the sensitivity changes to α- and β-adrenoceptor agonists after chronic DMI treatment; 2) exogenous NA reaching neuronal, but not muscular adrenoceptors, is affected by neuronal uptake mechanisms; 3) NA released by adrenergic terminals undergoes neuronal uptake and is controlled by α2-autoreceptors.
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References
Alberts P, Stjärne L (1982) Facilitation and muscarinic and α-adrenergic inhibition of the secretion of [3H]-acetylcholine and [3H]-noradrenaline from guinea-pig ileum myenteric nerve terminals. Acta Physiol Scand 116:83–92
Beam L, Bianchi C, Crema A (1969) The effect of catecholamines and sympathetic stimulation on the release of acetylcholine from the guinea-pig colon. Br J Pharmacol 36:1–17
Beam L, Bianchi C, Siniscalchi A, Sivilotti L, Tanganelli S, Veratti E (1984) Different approaches to study acetylcholine release; endogenous ACh versus tritium efflux. Naunyn-Schmiedeberg's Arch Pharmacol 328:119–126
Blandizzi C, Doda M, Tarkovacs G, Del Tacca M, Vizi ES (1991) Functional evidence that acetylcholine release from Auerbach's plexus of guinea-pig ileum is modulated by α2A-adrenoceptor subtype. Eur J Pharmacol 205:311–313
Bond RA, Charlton KG, Clarke DE (1986) Responses to norepinephrine resistant to inhibition by alpha and beta adrenoceptor antagonists. J Pharmacol Exp Ther 236:408–415
Boullin DJ, Costa E, Brodie BB (1967) Evidence that blockade of adrenergic receptors causes overflow of norepinephrine in cat's colon after nerve stimulation. J Pharmacol Exp Ther 157:125–134
Bowman WC, Hall TM (1970) Inhibition of rabbit intestine mediated by α- and β-adrenoceptive receptors. Br J Pharmacol 38:399–415
Crews FT, Smith CB (1978) Presynaptic alpha-receptor subsensitivity after long-term antidepressant treatment. Science 202:322–324
Crews FT, Smith CB (1980) Potentiation of response to adrenergic nerve stimulation in isolated rat atria during chronic tricyclic antidepressant administration. J Pharmacol Exp Ther 215:143–149
Daniel W, Danek L, Janczar L, Nocon H, Melzacka M (1991) Regional distribution of imipramine, desipramine and specific [3H] desipramine binding sites in the rat brain after acute and chronic treatment with imipramine. J Pharm Pharmacol 43:31–35
Duncan GE, Paul IA, Powell KR, Fassberg JB, Stumpf WE, Breese GR (1989) Neuroanatomically selective down-regulation of beta adrenergic receptors by chronic imipramine treatment: relationships to the topography of [3H] imipramine and [3H] desipramine binding sites. J Pharmacol Exp Ther 248:470–477
El Mas M, Hughes IE (1990) Effect of blockade of noradrenaline re-uptake on evoked tritium overflow from mouse vasa deferentia and rat cortex slices. Br J Pharmacol 101:762–768
Finberg JPM, Tal A (1985) Reduced peripheral presynaptic adrenoceptor sensitivity following chronic antidepressant treatment in rats. Br J Pharmacol 84:609–617
Frigo GM, Lecchini S (1970) An improved method for studying the peristaltic reflex in the isolated colon. Br J Pharmacol 39:346–356
Frigo GM, Lecchini S, Marcoli M, Tonini M, D'Angelo L, Crema A (1984) Changes in sensitivity to the inhibitory effects of adrenergic agonists on intestinal motor activity after chronic sympathetic denervation. Naunyn-Schmiedeberg's Arch Pharmacol 325: 145–152
Furness JB, Costa M (1980) Types of nerves in the enteric nervous system. Neuroscience 5:1–20
Gabella G (1972) Fine structure of the myenteric plexus in the guinea-pig ileum. J Anat 111:69–97
Gabella G (1979) Innervation of gastrointestinal tract. Int Rev Cytol 59:129–193
Garcia-Sevilla JA, Zubieta JK (1986) Activation and desensitization of presynaptic α2-adrenoceptors after inhibition of neuronal uptake by antidepressant drugs in the rat vas deferens. Br J Pharmacol 89:673–683
Gatti G, Marcoli M, Lecchini S, Frigo GM, Crema A (1987 a) Evidence of the involvemente of α2-auto- and α2-heteroceptors in the adrenergic control of intestinal motor activity. Pharmacol Res Commun 19:979–980
Gatti G, De Pond F, D'Angelo L, Caravaggi M, Lecchini S, Frigo GM, Crema A (1987b) A simple analytical method for determining catecholamine release from enteric neurons: an HPLC technique employing coulometric detection. It J Gastroenterol 19:282–284
Gillespie JS, Khoyi MA (1977) The site and receptors responsible for the inhibition by sympathetic nerves of intestinal smooth muscle and its parasympathetic motor nerves. J Physiol 267:767–789
Gonçalves J, Guimarães S (1991) Influence of neuronal uptake on pre-and postjunctional effects of alpha adrenoceptor agonists in tissues with noradrenaline-ATP cotrasmission. Naunyn-Schmiedeberg's Arch Pharmacol 344:532–537
Gordon-Weeks PR (1982) Noradrenergic and non-noradrenergic nerves containing small granular vesicles in Auerbach's plexus of the guinea-pig: evidence against the presence of noradrenergic synapses. Neuroscience 7:2925–2936
Govier WC, Sugrue MF, Shore PA (1969) On the inability to produce supersensitivity to catecholamines in intestinal smooth muscle. J Pharmacol Exp Ther 165:71–77
Hall H, Ross SB, Sallemark M (1984) Effect of destruction of central noradrenergic and serotonergic nerve terminals by systemic neurotoxins on the long-term effects of antidepressants on β-badrenoceptors and 5-HT2 binding sites in the rat cerebral cortex. J Neural Transm 59:9–23
Heal DJ, Prow MR, Buckett WR (1991) Determination of the role of noradrenergic and 5-hydroxytryptaminergic neurones in postsynaptic α2-adrenoceptor desensitization by desipramine and ECS. Br J Pharmacol 103:1865–1870
Hertting G, Axelrod J, Whitby LG (1961) Effects of drugs on the uptake and metabolism of [3H]-norepinephrine. J Pharmacol Exp Ther 134:146–153
Hong KW, Rhim BY, Lee WS (1986) Enhancement of central and peripheral alpha1-adrenoceptor sensitivity and reduction of alpha2-adrenoceptor sensitivity following chronic imipramine treatment in rats. Eur J Pharmacol 29:275–283
Kalsner S (1983) Yohimbine and prolongation of stimulation pulse duration alter similarly [3H]-transmitter efflux in heart: an alternative to the negative feedback hypothesis. Br J Pharmacol 79:985–992
Keith RA, Salama AI (1987) Inhibition of presynaptic alpha-2 adrenoceptor and opioid receptor agonist responses in the rat vas deferens by chronic imipramine treatment. Naunyn-Schmiedeberg's Arch Pharmacol 335:412–419
Keith RA, Howe BB, Salama AI (1986) Modulation of peripheral beta-1 and alpha-2 receptor sensitivities by the administration of the tricyclic antidepressant, imipramine, alone or in combination with alpha-2 antagonists to rats. J Pharmacol Exp Ther 236:356–363
Lacroix D, Blier P, Curer O, De Montigny C (1991) Effect of long-term desipramine administration on noradrenergic neurotransmission: electrophysiological studies in the rat brain. J Pharmacol Exp Ther 257:1081–1090
Langer SZ (1980) Presynaptic regulation of the release of cathecolmines. Pharmacol Rev 32:337–362
Lee CY (1970) Adrenergic receptors in the intestine. In: Bülbring E, Brading AF, Jones AW, Tomita T (eds) Smooth muscle. Clowes, London, pp 549–557
Llewellyn-Smith IJ, Wilson AJ, Furness JB, Costa M, Rusch RA (1981) Ultrastructural identification of noradrenergic axons and their distribution within the enteric plexuses of the guinea-pig small intestine. J Neurocytol 10:331–332
Lotti VJ, Chang RSL, Kling P (1981) Pre- and postsynaptic adrenergic activation by norepinephrine reuptake inhibitors in the field-stimulated rat vas deferens. Life Sci 29:633–639
Manber L, Gershon MD (1979) A reciprocal adrenergic-cholinergic axoaxonicsynapse in the mammalian gut. Am J Physiol 236: E738-E745
Marcoli M, Lecchini S, De Ponti F, D'Angelo L, Crema A, Frigo GM (1985) Subsensitivity of enteric cholinergic neurones to α2-adrenoceptor agonists after chronic sympathetic denervation. Naunyn-Schmiedeberg's Arch Pharmacol 329:271–277
Marcoli M, De Ponti F, Lecchini S, Crema A, Frigo GM (1989) [3H] acetylcholine release from the guinea-pig distal colon: comparison with ileal [3H] acetylcholine release and effect of adrenoceptor stimulation. J Pharm Pharmacol 41:824–828
Marshall I (1983) Stimulation-evoked release of [3H]-noradrenaline by 1, 10 or 100 pulses and its modification through presynaptic alpha 2-adrenoceptors. Br J Pharmacol 78:221–231
Menkes DB, Aghajanjan GK, McCall RB (1980) Chronic antidepressant treatment enhances α-adrenergic and serotonergic responses in the facial nucleus. Life Sci 27:45–55
Newman M, Lerer B (1989) Post-receptor-mediated increases in adenylate cyclase activity after chronic antidepressant treatment: relationship to receptor desensitization. Eur J Pharmacol 162:345–352
Nowak G (1989) Long-term effect of antidepressant drugs and electroconvulsive shock (ECS) on cortical α1-adrenoceptors following destruction of dopaminergic nerve terminals. Pharmacol Toxicol 64:469–470
Paton WDM, Vizi ES (1969) The inhibitory action of noradrenaline and adrenaline on acetylcholine output by guinea-pig ileum longitudinal muscle strips. Br J Pharmacol 35:10–28
Pleus RC, Shreve PE, Towes ML, Bylund DB (1993) Down-regulation of alpha2-adrenoceptor subtypes. Eur J Pharmacol 15:181–185
Reese JH, Cooper JR (1984) Noradrenergic inhibition of the nicotinically-stimulated release of acetylcholine from guinea-pig ileal synaptosomes. Biochem Pharmacol 33:1145–1147
Slack BE (1986) Pre and postsynaptic actions of noradrenaline and clonidine on myenteric neurons. Neuroscience 19:1303–1309
Shreve PE, Toews ML, Bylund DB (1991) α2A- and α2C-Adrenoceptor subtypes are differently down-regulated by norepinephrine. Eur J Pharmacol 207:275–276
Starke K (1977) Regulation of noradrenaline release by presynaptic receptor systems. Rev Physiol Biochem Pharmacol 77:1–24
Starke K, Göthert M, Kilberger H (1989) Modulation of neurotransmitter release by prsynaptic autoreceptors. Physiol Rev 69:864–989
Sugrue MF (1983) Chronic antidepressant therapy and associated changes in central monaminergic receptor functioning. Pharmacol Ther 21:1–33
Sulser F, Vetulani J, Mobley PL (1978) Mode of action of antidepressant drugs. Biochem Pharmacol 27:257–261
Tallarida RJ, Murray RB (1987) Manual of pharmacologic calculations with computer programs. Springer, New York Berlin Heidelberg
Tanaka T, Starke K (1979) Binding of [3H]-clonidine to an α-adrenoceptor in membranes of guinea-pig ileum. Naunyn-Schmiedeberg's Arch Pharmacol 309:207–215
Taneja DT, Clarke DE (1992) Evidence for a noradrenergic innervation to “atypical” beta adrenoceptors (or putative beta-3 adrenoceptors) in the ileum of guinea pig. J Pharmacol Exp Ther 260:192–200
Trendelenburg U (1972) Factors influencing the concentration of catecholamines at the receptors. In: Blaschko H, Muscholl E (eds) Catecholamines. Handbook of experimental pharmacology, vol. 33. Springer, Berlin Heidelberg New York, pp 726–762
Vetulani J, Sulser F (1975) Action of various antidepressant treatments reduces reactivity of noradrenergic cyclic AMP-generating system in limbic forebrain. Nature 257:495
Vizi ES (1979) Presynaptic modulation of neurochemical transmission. Progr Neurobiol 12:181–290
Wessler I, Kilbinger H (1986) Release of [3H] acetylcholine from a modified rat phrenic nerve-hemidiaphragm preparation. Naunyn-Schmiedeberg's Arch Pharmacol 334:357–364
Westfall TC (1977) Local regulation of adrenergic neurotransmission. Physiol Rev 57:659–728
Westfall TC (1984) Evidence that noradrenergic transmitter release is regulated by presynaptic receptors. Fed Proc 43:1352–1357
Wikberg J (1977) Localization of adrenergic receptors in guinea-pig ileum and rabbit jejunum to cholinergic neurones and to smooth muscle cells. Acta Physiol Scand 99:190–207
Wikberg JES, Lefkowitz RJ (1982) Alpha2 adrenergic receptors are located prejunctionally in the Auerbach's plexus of the guinea-pig small intestine: direct demonstration by radioligand binding. Life Sci 31:2899–2905
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Correspondence to: G. M. Frigo at the above address
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Marino, F., Marcoli, M., De Ponti, F. et al. Effect of desipramine-induced blockade of neuronal uptake mechanisms on adrenoceptor-mediated responses in the guinea-pig colon. Naunyn-Schmiedeberg's Arch Pharmacol 350, 499–506 (1994). https://doi.org/10.1007/BF00173019
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DOI: https://doi.org/10.1007/BF00173019