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Effects of human middle ear effusions on the mucociliary system of the tubotympanum in the guinea pig

European Archives of Oto-Rhino-Laryngology volume 252, pages 35–41 (1995)Cite this article

Abstract

Effusion fluid resulting from otitis media contains a variety of inflammatory substances. This middle ear effusion (MEE) may be itself affect the mucosa of the tubotympanum and contribute to chronicity of the condition. The present study was designed to elucidate in vitro and in vivo effects of MEEs on the mucosa of the guinea pig tubotympanum. The results obtained demonstrated that the ciliary activity of the eustachian tube was reduced in the presence of human MEEs. This activity decreased to approximately 80% in the presence of serous MEE at 48 h and was 60% after exposure to mucoid MEE. Intratympanic inoculation of human MEEs resulted in accumulation of a serous effusion in the tympanic cavity. Histologic study of the tubotympanum in inoculated animals demonstrated mucociliary dysfunction as well as a general inflammatory process and increased vascular permeability. This damage was more prominent following inoculation with the mucoid MEEs. The ciliary depression and inflammation found in the tubotympanum suggest that the pathologic nature of MEEs may be, at least partially, responsible for the chronicity of otitis media.

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References

  1. Berger G, Hawke M, Proops DW, Ranadive NS, Wong D (1984) Histamine levels in middle ear effusions. Acta Otolaryngol (Stockh) 98:385–390

    Google Scholar 

  2. Cauwenberge P, Bernstein J (1987) Inflammatory mediators in middle ear diseases. In: Bernstein J, Ogra P (eds) Immunology of the ear. Raven Press, New York, pp 338–339

    Google Scholar 

  3. DeMaria TF, Prior RB, Briggs BR, Lim DJ (1984) Endotoxin in middle-ear effusions from patients with chronic otitis media with effusion. J Clin Microbiol 20:15–17

    Google Scholar 

  4. Esaki Y, Ohashi Y, Furuya H, Sugiura Y, Ohno Y, Nakai Y (1991) Histamine-induced mucociliary dysfunction and otitis media with effusion. Acta Otolaryngol (Stockh) [Suppl] 486: 116–134

    Google Scholar 

  5. Jinnin T, Dake Y, Kusumoto T, Tabata T (1985) Quantitative determination of bacterial endotoxin in middle ear effusions by chromogenic substrate methods. Auris Nasus Larynx (Tokyo) 12 [Suppl]:97–99

    Google Scholar 

  6. Karja J, Nuutinen J, Karjalainen P (1983) Mucociliary function in children with secretory otitis media. Acta Otolaryngol (Stockh) 95:544–546

    Google Scholar 

  7. Lim DJ, Birck H (1971) Ultrastructural pathology of the middle ear mucosa in serous otitis media. Ann Otol Rhinol Laryngol 80:838–853

    Google Scholar 

  8. Moore GE, Gerner RE, Franklin HA (1967) Culture of normal human leukocytes. JAMA 199:519–524

    Google Scholar 

  9. Ohashi Y, Nakai Y (1991) Current concept of mucociliary dysfunction in otitis media with effusion. Acta Otolaryngol (Stockh) [Suppl] 486:149–161

    Google Scholar 

  10. Ohashi Y, Nakai Y, Zushi K, Muraoka M, Harada H (1983) Functional and morphological studies on chronic sinusitis mucous membrane. Acta Otolaryngol (Stockh) [Suppl] 397:3–59

    Google Scholar 

  11. Ohashi Y, Nakai Y, Kihara S (1985) Ciliary activity of the middle ear lining in guinea pigs. Ann Otol Rhinol Laryngol 94:419–423

    Google Scholar 

  12. Oashi Y, Nakai Y, Ikeoka H, Koshimo H (1986) Ciliary activiy in the in vitro tubotympanum. Arch Otorhinolaryngol 243:317–319

    Google Scholar 

  13. Ohashi Y, Nakai Y, Ikeoka H, Koshimo H, Esaki Y (1987) Effects of bacterial endotoxin on the ciliary activity in the in vitro middle ear mucosa. Acta Otolaryngol (Stockh) 104:495–499

    Google Scholar 

  14. Ohashi Y, Nakai Y, Kihara S, Ikeoka H (1987) Effects of Staphylococcus aureus on the ciliary activity of the middle ear lining. Ann Otol Rhinol Laryngol 96:225–228

    Google Scholar 

  15. Ohashi Y, Nakai Y, Furuya H, Esaki Y, Ikeoka H, Kato S, Kato M (1989) Mucociliary disease of the middle ear during experimental otitis media with effusion induced by bacterial endotoxin. Ann Otol Rhinol Laryngol 98:479–484

    Google Scholar 

  16. Ohashi Y, Nakai Y, Esaki Y, Ohno Y, Sugiura Y, Okamoto H (1991) Influenza A virus-induced otitis media and mucociliary dysfunction in the guinea pig. Acta Otolaryngol (Stockh) [Suppl] 486:135–148

    Google Scholar 

  17. Rhee C-K, Jung TK (1993) Platelet activating factor (PAF)-induced experimental otitis media with effusion and PAF-antagonist in chinchillas. In: Lim DJ, Bluestone CD, Klein JO, Nelson JD, Ogra PL (eds) Recent advances in otitis media. Dekker, New York, pp 482–485

    Google Scholar 

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Affiliations

  1. Department of Otolaryngology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno, 545, Osaka, Japan

    Y. Ohashi, Y. Nakai, Y. Ohno, Y. Sugiura & H. Okamoto

Authors
  1. Y. Ohashi
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  2. Y. Nakai
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  3. Y. Ohno
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  4. Y. Sugiura
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  5. H. Okamoto
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Ohashi, Y., Nakai, Y., Ohno, Y. et al. Effects of human middle ear effusions on the mucociliary system of the tubotympanum in the guinea pig. Eur Arch Otorhinolaryngol 252, 35–41 (1995). https://doi.org/10.1007/BF00171438

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  • DOI: https://doi.org/10.1007/BF00171438

Key words

  • Otitis media with effusion Tubotympanum
  • Mucociliary system
  • Guinea pig