Applied Microbiology and Biotechnology

, Volume 33, Issue 1, pp 48–50 | Cite as

Co-immobilization of amyloglucosidase and pullulanase for enhanced starch hydrolysis

  • Ajoy C. Chakrabarti
  • Kenneth B. Storey


Amyloglucosidase and pullulanase were co-immobilized using a hydrophilic polyurethane foam (Hypol® 2002). The combined amyloglucosidase and pullulanase activity of the immobilized enzyme was 32.2% ± 1.7% relative to the non-immobilized enzyme. The co-immobilized enzymes were capable of using a variety of glycogen and starch substrates. Co-immobilization of amyloglucosidase and pullulanase increased the glucose yield 1.6-fold over immobilized amyloglucosidase alone. No decrease in activity was observed after 4 months storage for the co-immobilized enzymes. The results suggest that co-immobilization of amyloglucosidase and pullulanase in polyurethane foams is a potentially useful approach for commercial starch hydrolysis.


Glucose Enzyme Hydrolysis Starch Foam 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Ajoy C. Chakrabarti
    • 1
  • Kenneth B. Storey
    • 1
  1. 1.Institute of Biochemistry and Department of BiologyCarleton UniversityOntarioCanada

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