Summary
Approximately 600 strains of various bacteria and actinomycetes were tested for their ability to hydroxylate o-acetyltoluidide (AT) at the 4'-position. Nocardia asteroides IFO 3384 and N. globerula IFO 13510 were selected as the best strains to catalyse the hydroxylation of AT. The product was isolated from culture] broth and identified as 4'-hydroxy-o-acetyltoluidide (4'-HAT). When AT was added during the course of cultivation of N. asteroides IFO 3384 and N. globerula IFO 13510, final concentrations of 1.0 mg/ml (a 23% molar conversion yield) and 1.5 mg/ml 4'-HAT (a 34% molar conversion yield) were obtained, respectively, from AT after 7 days cultivation. On the other hand, when resting cells of N. globerula IFO 13510 were used, the concentration of 4'-HAT attained was 4.4 mg/ml, a 69% molar conversion yield, after 4 days reaction.
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Nagasawa, T., Yoshioka, H. & Yamada, H. Microbial conversion of o-acetyltoluidide into 4′-hydroxy-o-acetyltoluidide. Appl Microbiol Biotechnol 34, 325–329 (1990). https://doi.org/10.1007/BF00170052
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DOI: https://doi.org/10.1007/BF00170052