Summary
The cytosolic fraction of N1E-115 neuroblastoma cells catalysed the l-arginine- and NADPH-dependent formation of a substance that relaxed endothelium-denuded strips of rabbit aorta. Relaxations in response to this substance were enhanced in the presence of superoxide dismutase. Nω-Nitro-l-arginine and NG-monomethyl-l-arginine, two inhibitors of EDRF synthesis, markedly attenuated the relaxations. Hemoglobin, a scavenger of EDRF, and methylene blue, an inhibitor of soluble guanylate cyclase, completely abolished the relaxation to N1E-115 cytosol. In contrast, the cyclo-oxygenase inhibitor indomethacin did not alter the relaxations. These data demonstrate that the cytosol of a neuronally-derived cell line is able to synthesize a substance with pharmacological properties similar to EDRF.
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This work was supported in part by Research Grants AM 30787 and HL 28474 from the National Institutes of Health, USA
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Förstermannr, U., Ishii, K., Gorsky, L.D. et al. The cytosol of N1E-115 neuroblastoma cells synthesizes an EDRF-like substance that relaxes rabbit aorta. Naunyn-Schmiedeberg's Arch Pharmacol 340, 771–774 (1989). https://doi.org/10.1007/BF00169689
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DOI: https://doi.org/10.1007/BF00169689