Advertisement

Applied Microbiology and Biotechnology

, Volume 35, Issue 5, pp 651–655 | Cite as

Magnetite formation by a magnetic bacterium capable of growing aerobically

  • Tadashi Matsunaga
  • Toshifumi Sakaguchi
  • Fumihiko Tadakoro
Applied Microbial and Cell Physiology

Summary

A helically shaped magnetic bacterium was isolated from freshwater sediment and a pure culture was obtained. The growth medium contained succinate, nitrate and ferric malate as the carbon, nitrogen and iron sources, respectively. The magnetic bacterium, designated AMB-1, was able to grow in free gaseous exchange with an air atmosphere. When cells were grown aerobically on agar, oxidase activity was present, and white non-magnetic colonies, which did not show catalase activity, were formed. The stationary phase of growth was reached 4–5 days later at a cell concentration of 1.4×109 cells/ml in liquid culture when an initial cell concentration of 105 cells/ml was employed. After ultrasonic disruption of harvested cells, 2.6 mg bacterial magnetite was obtained from a 11 culture.

Keywords

Magnetite Catalase Gaseous Exchange Succinate Cell Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Balkwill DL, Maratea D, Blakemore RP (1980) Ultrastructure of a magnetotactic spirillum. J Bacteriol 141:1399–1408Google Scholar
  2. Bazylinski DA, Blakemore RP (1983) Denitrification and assimilatory nitrate reduction Aquaspirillum magnetotacticum. Appl Environ Microbiol 46:1118–1124Google Scholar
  3. Bazylinski DA, Frankel RB, Jannasch HW (1989) Anaerobic magnetite production by marine, magnetotactic bacterium. Nature 334:518–519Google Scholar
  4. Blakemore RP, Maratea D, Wolfe S (1979) Isolation and pure culture of a freshwater magnetic spirillum in chemically defined medium. J Bacteriol 140:720–729Google Scholar
  5. Blakemore RP, Short KA, Bazylinski DA, Rosenblatt C, Frankel RB (1985) Microaerobic conditions are required for magnetite formation within Aquaspirillum magnetotacticum. Geomicrobiol J 4:53–71Google Scholar
  6. Farina F, Esquivel DMS, Lins de Barros HGP (1990) Magnetic iron-sulphur crystals from a magnetotactic microorganism. Nature 343:256–258Google Scholar
  7. Fassbinder JWE, Stanjek H, Vali H (1990) Occurrence of magnetic bacteria in soil. Nature 343:161–163Google Scholar
  8. Funaki M, Sakai H, Matsunaga T (1989) Identification of the magnetic poles on strong magnetic grains from meteorites using magnetotactic bacteria. J Geomag Geoelectr 41:77–87Google Scholar
  9. Mann S, Sparks NHC, Frankel RB, Bazylinski DA, Jannasch HW (1990) Biomineralization of ferrimagnetic greigite (Fe3S4) and iron pyrite (FeS2) in a magnetotactic bacterium. Nature 343:258–261Google Scholar
  10. Matsunaga T, Kamiya S (1987) Use of magnetic particles isolated from magnetotactic bacteria for enzyme immobilization. Appl Microbiol Biotechnol 26:328–332Google Scholar
  11. Matsunaga T, Hashimoto K, Nakamura N, Nakamura K, Hashimoto S (1989) Phagocytosis of bacterial magnetite by leucocytes. Appl Microbiol Biotechnol 31:401–405Google Scholar
  12. Towe K, Moench TT (1981) Electron-optical characterization of bacterial magnetite. Earth Sci Lett 52:213–220Google Scholar
  13. Wolin EA, Wolin MJ, Wolfe RS (1963) Formation of methane by bacterial extracts. J Biol Chem 238:2882–2886Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Tadashi Matsunaga
    • 1
  • Toshifumi Sakaguchi
    • 1
  • Fumihiko Tadakoro
    • 1
  1. 1.Department of BiotechnologyTokyo University of Agriculture and TechnologyTokyoJapan

Personalised recommendations