Applied Microbiology and Biotechnology

, Volume 35, Issue 5, pp 651–655 | Cite as

Magnetite formation by a magnetic bacterium capable of growing aerobically

  • Tadashi Matsunaga
  • Toshifumi Sakaguchi
  • Fumihiko Tadakoro
Applied Microbial and Cell Physiology


A helically shaped magnetic bacterium was isolated from freshwater sediment and a pure culture was obtained. The growth medium contained succinate, nitrate and ferric malate as the carbon, nitrogen and iron sources, respectively. The magnetic bacterium, designated AMB-1, was able to grow in free gaseous exchange with an air atmosphere. When cells were grown aerobically on agar, oxidase activity was present, and white non-magnetic colonies, which did not show catalase activity, were formed. The stationary phase of growth was reached 4–5 days later at a cell concentration of 1.4×109 cells/ml in liquid culture when an initial cell concentration of 105 cells/ml was employed. After ultrasonic disruption of harvested cells, 2.6 mg bacterial magnetite was obtained from a 11 culture.


Magnetite Catalase Gaseous Exchange Succinate Cell Concentration 
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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Tadashi Matsunaga
    • 1
  • Toshifumi Sakaguchi
    • 1
  • Fumihiko Tadakoro
    • 1
  1. 1.Department of BiotechnologyTokyo University of Agriculture and TechnologyTokyoJapan

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