Applied Microbiology and Biotechnology

, Volume 38, Issue 1, pp 115–116 | Cite as

Expression and overproduction of glucose oxidase in Aspergillus niger

  • Fadel A. Sharif
  • N. Gürdal Alaeddinoglu
Applied Genetics and Regulation Short Contribution


This report describes the expression of cloned glucose oxidase gene (god) in glucose-oxidase-deficient mutants (God) of Aspergillus niger NRRL-3, the use of this gene for the elevation of glucose oxidase (GOD) productivity in the parental strain, and the further improvement of GOD production by subjecting the transformants to nitrous acid mutagenesis.


Glucose Nitrous Acid Aspergillus Aspergillus Niger Parental Strain 
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  1. Bigelis R (1985) On primary metabolism and industrial fermentations. In: Bennet JW, Lasure LL (eds) Gene manipulations in fungi. Academic Press, London, pp 357–401Google Scholar
  2. Campbell EJ, Unkles SE, Macrid JA, Hondel C van den, Contreras R, Kinghorn JR (1989) Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase. Curr Genet 16:53–56Google Scholar
  3. Döppner T, Hatmeir W (1984) Glucose oxidation by modified mould mycelium. Starch/Stärke 36:283–287Google Scholar
  4. Fiedurek J, Rogalski J, Ilczuk Z, Leonowicz A (1986) Screening and mutagenesis for the improvement of glucose oxidase production. Enzyme Microb Technol 8:734–736Google Scholar
  5. Fiedurek J, Rogalski J, Ilczuk Z (1990) Intensification of glucose oxidase synthesis with Aspergillus niger by the way of multi-stage mutagenesis. Acta Biotechnol 10:371–376Google Scholar
  6. Frederick KR, Tung J, Emerick RS, Masiaz FR, Chamberlain SH, Vasavada A, Rosenberg S (1990) Glucose oxidase from Aspergillus niger. Cloning, gene sequence, secretion from Saccharomyces cerevisiae and kinetic analysis of a yeast derived enzyme. J Biol Chem 265:3793–3802Google Scholar
  7. Hanahan D (1985) In: Glover MD (ed) DNA cloning, vol I. IRL, Oxford, pp 109–135Google Scholar
  8. Kelly JM, Hynes MJ (1985) Transformation of Aspergillus niger by amdS gene of Aspergillus nidulans. EMBO J 4:475–479Google Scholar
  9. Keston AS (1956) Specific colorimetric enzymatic analytical reagents for glucose. Abstract of papers, 12th Meeting, American Chemical Society, Dallas (TX), p 310Google Scholar
  10. Kriechbaum M, Heilmann HJ, Wientjes FJ, Hahn M, Jany KD, Gassen HJ, Sharif FA, Alaeddinoglu GN (1989) Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3. FEBS Lett 255:63–66Google Scholar
  11. Kundu PN, Das A (1985) A note on crossing experiments with Aspergillus niger for the production of calcium gluconate. J Appl Bacteriol 59:1–5Google Scholar
  12. Markwell J, Frakes LG, Brott EC, Osterman J, Wagner FW (1989) Aspergillus niger mutants with increased glucose oxidase production. Appl Microbiol Biotechnol 30:166–169Google Scholar
  13. Messing J, Gronenborn B, Müller-Hill B, Hofschneider PH (1977) Filamentous coliphage M13 as a cloning vehicle: insertion of a HindIII fragment of the lac regulatory region in M13 replicative form in vitro. Proc Natl Acad Sci USA 74:3642–3646Google Scholar
  14. Punt PJ, Oliver RP, Digemense MA, Pouwels PH, Hondel C van den (1987) Transformation of Aspergillus niger based on hygromycin B resistance marker from E. coli. Gene 56:117–124Google Scholar
  15. Swart K, Vondervoort P van den, Witteveen CFB, Visser J (1990) Genetic localization of a series of genes affecting glucose oxidase levels in Aspergillus niger. Curr Genet 18:435–439Google Scholar
  16. Tilburn J, Scazzochino C, Taylor GG, Zabicky-Zissman JH, Lockington RA, Davies RW (1983) Transformation by integration in Aspergillus nidulans. Gene 26:205–221Google Scholar
  17. Witteveen CFB, Vondervoort P van den, Swart K, Visser J (1990) Glucose oxidase overproducing and negative mutants of Aspergillus niger. Appl Microbiol Biotechnol 33:734–736Google Scholar
  18. Wittington H, Williams SK, Bidgood K, Dodsworth N, Peberdy J, Dobson M, Hinchliffe E, Ballance DJ (1990) Expression of the Aspergillus niger glucose oxidase gene in A. niger, A. nidulans and Saccharomyces cerevisiae. Curr Genet 18:531–536Google Scholar
  19. Yelton MM, Hamer JE, Timberlake WE (1984) Transformation of Aspergillus nidulans by a trpC plasmid. Proc Natl Acad Sci USA 81:1470–1474Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Fadel A. Sharif
    • 1
  • N. Gürdal Alaeddinoglu
    • 1
  1. 1.Department of Biological SciencesMiddle East Technical UniversityAnkaraTurkey

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