Abstract
Differential effects of [Ca2+] on catecholamine release and biosynthesis in isolated bovine adrenal chromaffin cells were investigated. Carbamylcholine, an agonist of the nicotinic and muscarinic acetylcholine receptor, or Na+ deprivation in the incubation medium, stimulated catecholamine release and biosynthesis in these cells. The concentrations of extracellular [Ca2+] which stimulate catecholamine biosynthesis were less than those which stimulate catecholamine release. An increase in intracellular levels of free Ca2+ ([Ca2+]i) induced by Na+ deprivation was dependent on extracellular [Ca2+]. These results indicate that, in bovine adrenal chromafn cells, catecholamine biosynthesis is regulated by lower levels of [Ca2+]i than is catecholamine release.
This is a preview of subscription content, log in via an institution to check access.
References
Baker PF, Knight DE (1984) Calcium control of exocytosis in bovine adrenal medullary cells. Trends Neurosci 7:120–126
Castagna M, Takai Y, Kaibuchi K, Sano K, Kikkawa U, Nishizuka Y (1982) Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. J Biol Chem 257:7847–7851
Grynkiewicz G, Poenie M, Tsien RY (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260:3440–3450
Houchi H, Masuda Y, Ishimura Y, Ohuchi T, Murakumo Y, Oka M (1994a) Calcium efflux from cultured bovine adrenal chromaffin cells induced by bradykinin. Biochem Pharmacol 47:1309–1313
Houchi H, Masuda Y, Murakumo Y, Ishimura Y, Ohuchi T, Oka M (1994b) Muscarinic receptor-mediated calcium efflux from cultured bovine adrenal chromaffin cells. Biochem Pharmacol 48:1982–1985
Kelner KL, Levine RA, Morita K, Pollard HB (1985) A comparison of trihydroxyindole and HPLC/electrochemical methods for catecholamine measurement in adrenal chromaffin cells. Neurochem Int 7:373–378
Knight DE, Baker PF (1983) The phorbol ester TPA increases the affinity of exocytosis for calcium in leaky adrenal medullary cells. FEBS Lett 160:98–100
Masserano JM, Vulliet PR, Tank AW, Weiner N (1990) The role of tyrosine hydroxylase in the regulation of catecholamine synthesis. In: Trendelenburg U, Weiner N (eds) Catecholamines. (Handbook of experimental pharmacology series) Springer, Berlin Heidelberg New York, pp 427–469
Matsuoka M (1964) Function and metabolism of catecholamines in the brain. Jpn J Pharmacol 14:181–193
Mori T, Takai Y, Yu B, Takahashi J, Nishizuka Y, Fujikura T (1982) Specificity of the fatty acyl moieties of diacylglycerol for the activation of calcium-activated, phospholipid-dependent protein kinase. J Biochem 91:427–431
Nishizuka Y (1984) The role of protein kinase C in cell surface signal transduction and tumor promotion. Nature 312:315–321
Oka M, Isosaki M, Yanagihara N (1979) Isolated bovine adrenal medullary cells: studies on regulation of catecholamine synthesis and release. In: Usdin E, Kopin IJ, Barchas J (eds) Catecholamines: basic and clinical frontiers. Pergamon Press, Oxford, pp 70–72
Oka M, Isosaki M, Watanabe J (1981) Calcium flux and catecholamine release in isolated bovine adrenal medullary cells: Effects of nicotinic and muscarinic stimulation. In: Izumi F, Oka M, Kumakura K (eds) Advances in the biosciences: synthesis, storage and secretion of adrenal Catecholamines. Pergamon Press, Oxford, pp 29–36
Plevin R, Boarder MR (1988) Stimulation of formation of inositol phosphates in primary cultures of bovine adrenal chromaffin cells by angiotensin II, histamine, bradykinin and carbachol. J Neurochem 51:634–641
Stauderman KA, Press RM (1990) Different patterns of agonist-stimulated increases of 3H-inositol phosphate isomers and cytosolic Ca2+ in bovine chromaffin cells: comparison of the effects of histamine and angiotensin II. J Neurochem 54:946–953
Wada A, Yanagihara N, Izumi F, Sakurai S, Kobayashi H (1983) Trifluoperazine inhibits 45Ca2+ uptake and catecholamine secretion and synthesis in adrenal medullary cells. J Neurochem 40:481–486
Winkler H (1990) Occurrence and mechanism of exocytosis in adrenal medulla and sympathetic nerve. In: Trendelenburg U, Weiner N (eds) Catecholamines. (Handbook of experimental pharmacology series) Springer, Berlin Heidelberg New York, pp 43–118
Yamauchi T, Fujisawa H (1982) Phosphorylation of microtubule-associated protein 2 by calmodulin-dependent protein kinase (kinase II) which occurs only in the brain tissues. Biochem Biophys Res Commun 109:975–981
Yamauchi T, Nakata H, Fujisawa H (1981) A new activator protein that activates tryptophan 5-monooxygenase and tyrosine 3-monooxygenase in the presence of Ca2+-, calmodulin-dependent protein kinase. J Biol Chem 256:5404–5409
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Houchi, H., Yoshizumi, M., Ishimura, Y. et al. Differential requirements for Ca2+ concentrations for catecholamine release and biosynthesis in isolated bovine adrenal chromaffin cells. Naunyn-Schmiedeberg's Arch Pharmacol 353, 596–598 (1996). https://doi.org/10.1007/BF00169182
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00169182