Summary
The stimulating effect of cisapride on the motility of the digestive tract is well known. However, there are only a few studies on the influence of this drug on the absorptive or secretory activity of the colonic mucosa. In the present study, the ability of cisapride to alter the mural transport of water and electrolytes in the colon and its effects on mucus secretion and albumin permeation were studied.
The effects of cisapride on the rat colon in vivo were studied under different conditions, by means of an instillation of sodium acetate solution at pH 6.9, which induced absorption of water and electrolytes, and in two models of colonic secretion, one employing the instillation of an acetic acid solution at pH 2.9 and the other, an intravenous infusion of 5-hydroxytryptamine (serotonin) 45 μg · kg−1 · min−1 together with intracolonic instillation of sodium acetate.
Cisapride (i.v.), at a dose of 0.32 mg · kg−1, in rats whose colon was instilled with sodium acetate (pH 6.9) induced an increase in sodium absorption and a reduction in water absorption.
Cisapride (iv.), at doses of 0.32, 0.64 and 1.0 mg · kg−1, inhibited the secretion of water, Na+, Cl−, and mucus and the permeation of albumin induced by acetic acid instillation or by serotonin infusion. It is concluded that the effect of cisapride on the colonic mucosa varies in accordance with the functional mucosal conditions and that this action may be of clinical importance.
Similar content being viewed by others
References
Bandi JC, Rosembeck G, Celener D, Ledesma de Paolo MI, Bustos Fernandez L (1985) Cisapride-evoked 5HT2 receptor blockage inhibits acetic acid and 5-HT induced colonic secretion in the rat. Gastroenterology 96:A25
Beubler E, Burg G, Coupar IM, Hardcastle PT, Hardcastle J, Kitchin AJ (1989) 5 HT actions on intestinal fluid and electrolyte transport. Gut:A 1480
Bustos Fernandez L, Gonzalez E, Ledesma de Paolo MI, Celener D, Ogawa K (1976) Organic anions induce colonic secretion. Dig Dis 21:329–332
Costall B, Naylor RJ (1990) 5-Hydroxytryptamine: New Receptors and novel drugs for gastrointestinal motor disorders. Scand J Gastroenterol 25:769–787
Gonzalez E, Bernard C, Hamamura S, Caccamo D, Ledesma de Paolo MI, Kofoed A, Bustos Fernandez L (1985) Acetate anion in a model for the study of the rat absorptive and secretory function of the rat colon. Comunicaciones Biologicas 4:221–241
Hardcastle J, Hardcastle PT, Kelleher DK (1984) The action of R 51619 on transport processes in the rat small intestine. J Pharm Pharmacol 36:139–140
Hyden SA (1955) Turbidimetric method for the determination of PEG in biological material. Ann Swed Agric Coll 22:139–145
Inokuchi H, Kawai K, Takeuchi Y, Sano Y (1982) Immunohistochemical demonstration of EC cells in rat gastrointestinal tract. Histochemistry 74:453–456
Inokuchi H, Kawai K, Takeuchi Y, Sano Y (1983) Identification of EC cells in the human intestine: A comparative study between immunohistochemical and silver impregnation techniques. Histochemistry 79:9–16
Laurell CB (1966) Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem 15:45–52
Moriarty KJ, Higgs NB, Woodford M, Warhurst G, Turnberg LA (1986) Cisapride inhibits the secretory action of serotonin (5HT) in mammalian small intestine. Gut, A 1111
Moriarty KJ, Higgs NB, Woodford M, Warhurst G, Turnberg LA (1987) Inhibition of the effect of serotonin on rat ileal transport by cisapride: evidence in favour of the involvement of 5 HT2 receptors. Gut 28:844–848
Reyntjens A, Verlinden M, Schuurkes J, Van Nueten J, Janssen PAJ (1984) New approach to gastrointestinal motor dysfunction: non-antidopaminergic, non-cholinergic stimulation with cisapride. An introductory survey. Curr Ther Res 36:1029–1037
Schwörer H, Racké K, Kilbinger H (1987) Cholinergic modulation of the release of 5-hydroxytryptamine from the guinea pig ileum. Naunyn-Schmiedeberg's Arch Pharmacol 336:127–132
Schwörer H, Racké K, Kilbinger H (1989) Characterization of the muscarine receptors involved in the modulation of serotonin release from the vascularly perfused small intestine of guinea pig. Naunyn-Schmiedeberg's Arch Pharmacol 339:263–267
Schuurkes J, Van Nueten J (1988) Animal pharmacology of the gastrointestinal prokinetic, cisapride. In: Johnson AG, Lux G (eds) Progress in the treatment of gastrointestinal motility disorders: The role of cisapride. Excerpta Medica, Amsterdam, pp 11–22
Schuurkes J, Akkermans LMA, Van Nueten J (1984) Stimulating effects of cisapride on antroduodenal motility in the conscious dog. In.: Roman C (ed) Gastrointestinal motility. MTP Press, Lancaster, pp 95–102
Van Nueten J, Schuurkes J (1986) Non-cholinergic stimulation of the guinea pig colon by cisapride. Gastroenterology 90:A 1677
Zimmerman TN, Binder HJ (1984) Serotonin-induced alterations of colonic electrolytes transport in the rat. Gastroenterology 86:310–317
Author information
Authors and Affiliations
Additional information
Correspondence to D. Celener at the above address
Rights and permissions
About this article
Cite this article
Bandi, J.C., Rosembeck, G., De Paula, J.A. et al. Action of cisapride on rat colonic secretion. Naunyn-Schmiedeberg's Arch Pharmacol 348, 319–324 (1993). https://doi.org/10.1007/BF00169162
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00169162