Summary
The effect of the calcium entry blocker flunarizine on a high-potassium induced increase of intracellular free calcium was studied. The experiments were done with neurons isolated from rat dorsal root ganglia and loaded with the calcium-sensitive dye fura-2. The increase of calcium induced by 60 mmol/1 potassium was abolished after removal of extracellular calcium, was reversibly reduced by 50 μmol/l cadmium (76% inhibition), 50 μmol/1 nickel (25% inhibition) and 10 μmol/1 nifedipine (18°10 inhibition), and reversibly increased after removal of extracellular sodium (26% increase). The potassium induced increase of intracellular calcium is, therefore, mediated by transmembrane calcium influx, probably to a large extent through cadmium-sensitive calcium channels. Flunarizine (5 min incubation followed 1 min wash-out) reduced the amplitude of the high-potassium induced calcium increase in a dose-dependent manner (K d = 370 ± 100 nmol/l; mean ± SEM; n = 8), causing complete inhibition at a concentration of 10 μmol/1 in the majority of cells. Flunarizine (≥ 1 μmol/1) caused a reversible increase of the resting level of intracellular calcium in some cells, an effect which disappeared in the absence of extracellular calcium. The drug (1 μmol/1 had no influence on the time course of recovery of intracellular calcium subsequent to a rise induced by high-potassium or by the calcium ionophore A23187. It is concluded that flunarizine acts as an inhibitor of depolarization-mediated calcium influx. At a concentration of 1 μmol/1, the drug presumably has no effect on cellular calcium extrusion and/or sequestration mechanisms.
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Abiko H, Mizoi K, Suzuki J, Oba M, Yoshimoto T (1988) Cerebral protective effect of flunarizine in a canine model of cerebral ischaemia. Neurol Res 10:145–150
Akaike N, Kostyuk PG, Osipchuk YV (1989) Dihydropyridine-sensitive low-threshold calcium channels in isolated rat hypothalamic neurones. J Physiol (Lond) 412:181–195
Allen TJA, Baker PF (1986) Comparison of the effects of potassium and membrane potential on the calcium-dependent sodium efflux in squid axons. J Physiol (Lond) 378:53–76
Ashton D, Willems R, Marrannes R, Janssen P (1990) Extracellular ions during veratridine-induced neuronotoxicity in hippocampal slices: neuroprotective effects of flunarizine and tetrodotoxin. Brain Res 528:212–222
Benham CD, Evans ML, McBain CJ (1989) Inhibition of Ca-ATPase slows recovery from voltage-gated Cai load in neurons from rat dorsal root ganglia (in vitro). J Physiol (Lond) 415:21P
Carbone E, Lux HD (1987) Kinetics and selectivity of a low-voltage-activated calcium current in chick and rat sensory neurones. J Physiol (Lond) 386:547–570
Chiang DH, Wei JW (1987) Effects of calcium antagonists on (Na+/K+)-ATPase, Mg2+-ATPase and Ca2+-ATPase activities of rat cortical synaptosomes. Gen Pharmacol 18:563–567
de Hemptinne A, Marrannes R, Vanheel B (1982) Double-barreled intracellular pH electrode: construction and illustration of some results. In: Nucitelli R, Deamer DW (eds) Intracellular pH: Its measurement, regulation and utilization in cellular functions. Liss, New York, pp 7–19
De Ley G, Leybaert L (1993) Effect of flunarizine and methylprednisolone on functional recovery after experimental spinal injury. J Neurotr 10:25–35
De Ley G. Weyne J, Demeester G, Stryckmans K, Goethals P, Leusen I (1989) Streptokinase treatment versus calcium overload blockade in experimental thromboembolic stroke. Stroke 20:357–361
Deshpande JK, Wieloch T (1986) Flunarizine, a calcium entry blocker, ameliorates ischemic brain damage in the rat. Anesthesiology 64:215–224
Duchen MR, Valdeolmillos M, O'Neill SC, Eisner DA (1990) Effects of metabolic blockade on the regulation of intracellular calcium in dissociated mouse sensory neurones. J Physiol (Lond) 424:411–426
Dzurba A, Breier A, Slezak J, Stankovicova T, Vrbjar N, Ziegelhoffer A (1991) Influence of calcium antagonists on heart sarcolemmal Na+/K+-ATPase. Bratisl Lek Listy 92:155–158
Fox AP, Nowycky MC, Tsien RW (1987) Kinetic and pharmacological properties distinguishing three types of calcium currents in chick sensory neurones. J Physiol (Lond) 394:149–172
Grynkiewicz G, Poenie M, Tsien RY (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260:3440–3450
Harris RJ, Symon L, Branston NM, Bayhan M (1981) Changes in extracellular calcium activity in cerebral ischemia. J Cereb Blood Flow Metab 1:203–209
Hartmann A, van Zwieten PA (1989) Treatment of neurological disorders except migraine with calcium antagonists. In: van Zwieten PA (ed) Clinical aspects of calcium entry blockers. Karger, Basel New York, pp 155–193
Hess P (1990) Calcium channels in vertebrate cells. Annu Rev Neurosci 13:337–356
Langer GA (1982) Sodium-calcium exchange in the heart. Annu Rev Physiol 44:435–449
Leybaert L, de Hemptinne A (1989) Resting potential characteristics of rat dorsal root ganglion cells cultured in vitro. Arch Int Physiol Biochim 97:P67
Leybaert L, de Hemptinne A (1993) A voltage-clamp study of calcium currents in neurons freshly isolated from the dorsal root ganglion of adult rat. Arch Int Physiol Biochim ( in press)
Llinás R, Sugimoro M, Lin J-W, Cherksey B (1989) Blocking and isolation of a calcium channel from neurons in mammals and cephalopods utilizing a toxin fraction (FTX) from funnel-web spider poison. Proc Natl Acad Sci USA 86:1689–1693
Lugnier C, Follenius A, Gerard D, Stoclet JC (1984) Bepridil and flunarizine as calmodulin inhibitors. Eur J Pharmacol 98:157–158
Mela-Riker LM, Goss JR, Takashima K, Riker WK (1985) Opposite effects of flunarizine on brain mitochondrial and synaptosomal function. Proceedings of the symposium: Pharmacological stabilization of excitable membranes. Bratisl Lek Listy (abstract)
Michiels M, Hendriks F, Knaeps F, Woestenborghs R, Heykants J (1983) Absorption and tissue distribution of flunarizine in rats, pigs and dogs. Azneimittelforschung 33:1135–1142
Mintz IM, Venema VJ, Swiderek KM, Lee TD, Bean BP, Adams ME (1992) P-type calcium channels blocked by the spider toxin ω-Aga-IVA. Nature 355:827–829
Perrier ML, Scatton B, Benavides J (1992) Dihydropyridine- and ω-con-otoxin-resistant, neomycin-sensitive calcium channels mediate the depolarization-induced increase in internal calcium levels in cortical slices from immature rat brain. J Pharmacol Exp Ther 261:324–330
Plummer MR, Logothetis DE, Hess P (1989) Elementary properties and pharmacological sensitivities of calcium channels in mammalian peripheral neurons. Neuron 2:1453–1463
Press WH, Flannery BP, Teukolsky SA, Vetterling WT (1989) Numerical recipes in Pascal. Cambridge University Press, Cambridge 572–580
Terada K, Ohya Y, Kitamura K, Kuriyama H (1987) Actions of flunarizine, a Ca antagonist, on ionic currents in fragmented smooth muscle cells of the rabbit small intestine. J Pharmacol exp Ther 240:978–983
Thayer AS, Miller RJ (1990) Regulation of the intracellular calcium concentration in single rat dorsal root ganglion neurones in vitro. J Physiol (Lond) 425:85–115
Tytgat J, Pauwels PJ, Vereecke J, Carmeliet E (1991) Flunarizine inhibits a high-threshold inactivating calcium channel (N-type) in isolated hippocampal neurons. Brain Res 549:112–117
Wei JW Chiang DH (1986) Effects of calcium antagonists on KCl-evoked calcium uptake by rat cortical synaptosomes. Gen Pharmacol 17:261–265
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Leybaert, L., De Ley, G. & de Hemptinne, A. Effects of flunarizine on induced calcium transients as measured in fura-2-loaded neurons of the rat dorsal root ganglion. Naunyn-Schmiedeberg's Arch Pharmacol 348, 269–274 (1993). https://doi.org/10.1007/BF00169155
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DOI: https://doi.org/10.1007/BF00169155