Abstract
The effects of nitric oxide (NO) synthase inhibitors on the electrically evoked release of [3H]acetylcho-line were studied in guinea-pig myenteric plexus preparations preincubated with [3H]choline. NG-monomethyl-l-arginine (EC50 5.3 μmol l−1) and NG-nitro-l-arginine (EC50 1.3 μmol l−1) concentration-dependently increased the evoked release of [3H]acetylcholine without affecting the basal outflow. The facilitatory effect of NG-monomethyl-l-arginine was prevented by l-arginine but not by d-arginine. The results suggest that endogenous NO inhibits the depolarisation-evoked release of acetylcholine.
This is a preview of subscription content, log in via an institution to check access.
References
Alberts P, Ögren VR (1988) Interaction of forskolin with the effect of atropine on [3H] acetylcholine secretion in guinea-pig ileum myenteric plexus. J Physiol 395:441–451
Baccari MC, Bertini M, Calamai F (1993) Effects of l-NG-nitro arginine on cholinergic transmission in the gastric muscle of the rabbit. Neuroreport 4:1102–1104
Brave SR, Hobbs AJ, Gibson A, Tucker JF (1991) The influence of lNG-nitro-arginine on field stimulation induced contractions and acetylcholine release in guinea pig isolated tracheal smooth muscle. Biochem Biophys Res Commun 179:1017–1022
Calignano A, Whittle BJR, Dirosa M, Moncada S (1992) Involvement of endogenous nitric oxide in the regulation of rat intestinal motility in vivo. Eur J Pharmacol 229:273–276
Garthwaite J (1991) Glutamate, nitric oxide and cell-cell signalling in the nervous system. Trends Neurosci 14:60–67
Grider JR, Jin JG (1993) Vasoactive intestinal peptide release and l-citrulline production from isolated ganglia of the myenteric plexus: Evidence for regulation of vasoactive intestinal peptide release by nitric oxide. Neuroscience 54:521–526
Gustafsson LE, Wiklund CU, Wiklund NP, Persson MG, Moncada S (1990) Modulation of autonomic neuroeffector transmission by nitric oxide in guinea pig ileum. Biochem Biophys Res Commun 173:106–110
Kilbinger H, Nafziger M (1985) Two types of neuronal muscarine receptors modulating acetylcholine release from guinea-pig myenteric plexus. Naunyn Schmiedebergs Arch Pharmacol 328:304–309
Kilbinger H, Wolf D (1993) Nitric oxide inhibits the electrically evoked release of acetylcholine from guinea-pig myenteric plexus. Fundam Clin Pharmacol 7:367
Knudsen MA, Tottrup A (1992) A possible role of the l-arginine-nitric oxide pathway in the modulation of cholinergic transmission in the guinea-pig taenia coli. Br J Pharmacol 107:837–841
Lefebvre RA, De Vriese A, Smits GJM (1992) Influence of vasoactive intestinal polypeptide and NG-nitro-l-arginine methyl ester on cholinergic neurotransmission in the rat gastric fundus. Eur J Pharmacol 221:235–242
Llewellyn-Smith IJ, Song ZM, Costa M, Bredt DS, Snyder SH (1992) Ultrastructural localization of nitric oxide synthase immunoreactivity in guinea-pig enteric neurons. Brain Res 577:337–342
Mülsch A, Busse R (1990) NG-nitro-l-arginine (N5-[imino(nitroamino) methyl]-l-ornithine) impairs endothelium-dependent dilations by inhibiting cytosolic nitric oxide synthesis from l-arginine. Naunyn Schmiedebergs Arch Pharmacol 341:143–147
Prast H, Philippu A (1992) Nitric oxide releases acetylcholine in the basal forebrain. Eur J Pharmacol 216:139–140
Sanders KM, Ward SM (1992) Nitric oxide as a mediator of nonadrenergic noncholinergic neurotransmission. Am J Physiol 262:G379-G392
Sekizawa K, Fukushima T, Ikarashi Y, Maruyama Y, Sasaki H (1993) The role of nitric oxide in cholinergic neurotransmission in rat trachea. Br J Pharmacol 110:816–820
Snyder SH, Bredt DS (1991) Nitric oxide as a neuronal messenger. Trends Pharmacol Sci 12:125–127
Suzuki T, Nonaka H, Fujimoto K, Kawashima K (1993) Effects of physostigmine and some nitric oxide-cyclic GMP-related compounds on muscarinic receptor-mediated autoinhibition of hippocampal acetylcholine release. J Neurochem 60:2285–2289
Ward JK, Belvisi MG, Fox AJ, Miura M, Tadjkarimi S, Yacoub MH, Barnes PJ (1993) Modulation of cholinergic neural bronchoconstriction by endogenous nitric oxide and vasoactive intestinal peptide in human airways invitro. J Clin Invest 92:736–742
Wiklund CU, Olgart C, Wiklund NP, Gustafsson LE (1993a) Modulation of cholinergic and substance P-like neurotransmission by nitric oxide in the guinea-pig ileum. Br J Pharmacol 110:833–839
Wiklund CU, Wiklund NP, Gustafsson LE (1993b) Modulation of neuroeffector transmission by endogenous nitric oxide — a role for acetylcholine receptor-activated nitric oxide formation, as indicated by measurements of nitric oxide/nitrite release. Eur J Pharmacol 240:235–242
Wiklund NP, Leone AM, Gustafsson LE, Moncada S (1993c) Release of nitric oxide evoked by nerve stimulation in guinea-pig intestine. Neuroscience 53:607–611
Author information
Authors and Affiliations
Additional information
Correspondence to: H. Kilbinger at the above address
Rights and permissions
About this article
Cite this article
Kilbinger, H., Wolf, D. Increase by NO synthase inhibitors of acetylcholine release from guinea-pig myenteric plexus. Naunyn-Schmiedeberg's Arch Pharmacol 349, 543–545 (1994). https://doi.org/10.1007/BF00169145
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00169145