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Inhibitory effects of ovomacroglobulin on bacterial keratitis in rabbits

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Abstract

We studied the inhibitory effects of chicken egg-white ovomacroglobulin (ovoM) on keratitis induced by 56000-Da protease (56 KP) of Serratia marcescens and by elastase (PE) and alkaline protease (PAP) of Pseudomonas aeruginosa. The effects of ovoM on the serratial and pseudomonal keratitis in rabbits were also elucidated. In one model, four drops of 56 KP, PE, or PAP (1 mg/ml) were applied to wounded corneas of eight eyes. Thereafter, 80 μl ovoM (10 mg/ml) was dropped into four eyes and 0.01 M phosphate-buffed 0.15 M saline (pH 7.4) into the other eyes as a control. The other in vivo test system involved intrastromal injection of S. marcescens or P. aeruginosa, by which each sample (105–107 colony-forming units) mixed with ovoM was injected into one cornea and the other cornea received organisms without ovoM. OvoM completely inhibited the activity of these bacterial proteases in vitro and reduced corneal destruction in experimental keratitis in rabbits. In addition, greatly accelerated wound healing was observed.

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Authors and Affiliations

  1. Department of Ophthalmology, Kumamoto University Medical School, 860, Kumamoto, Japan

    Shin-ichi Miyagawa, Ryuji Kamata, Koki Matsumoto & Ryo-ichi Okamura

  2. Department of Microbiology, Kumamoto University Medical School, 860, Kumamoto, Japan

    Shin-ichi Miyagawa & Hiroshi Maeda

Authors
  1. Shin-ichi Miyagawa
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  2. Ryuji Kamata
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  3. Koki Matsumoto
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  4. Ryo-ichi Okamura
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  5. Hiroshi Maeda
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Additional information

Part of this work was presented at the 1989 meeting of the Association for Research in Vision and Ophthalmology in Sarasota, Florida

Offprint requests to: H. Maeda

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Miyagawa, Si., Kamata, R., Matsumoto, K. et al. Inhibitory effects of ovomacroglobulin on bacterial keratitis in rabbits. Graefe's Arch Clin Exp Ophthalmol 229, 281–286 (1991). https://doi.org/10.1007/BF00167885

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  • DOI: https://doi.org/10.1007/BF00167885

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