Abstract
In all, 21 diabetic and 23 non-diabetic patients underwent endothelial fluorophotometry before and at 4 days, 3 weeks and 6 weeks after phacoemulsification with posterior chamber intraocular lens (IOL: PMMA) implantation. After topical fluorescein instillation, fluorophotometry was performed using an automated computerized fluorophotometer. The aim of this study was to assess early postoperative endothelial damage quantitatively and to detect possible differences between diabetic and non-diabetic patients in that regard. Preoperatively, the endothelial permeability of diabetic patients did not differ from that of non-diabetic individuals (P = 0.5). At 4 days after phacoemulsiflcation with posterior chamber IOL implantation, endothelial permeability was significantly increased in both groups (P < 0.001), but significantly more so in diabetic patients (P < 0.001). The endothelial barrier function had fully recovered 3 weeks after surgery in non-diabetic subjects and 6 weeks after surgery in diabetic patients as well.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baum JP, Maurice DM, McCarey BE (1984) The active and passive transport of water across the corneal endothelium. Exp Eye Res 39:335–342
Bourne WM, Brubaker RF (1982) Decreased endothelial permeability in the iridocorneal endothelial syndrome. Ophthalmology 89:591–599
Bourne WM, Brubaker RF (1983) Decreased endothelial permeability in transplanted corneas. Am J Ophthalmol 96:362–367
Burns RR, Bourne WM, Brubaker RE (1981) Endothelial functions in patients with cornea guttata. Invest Ophthamol Vis Sci 20:77–80
Coakes R, Brubaker RF (1979) Method of measuring aqueous humor flow and corneal endothelial permeability using a fluorophotometry nomogram. Invest Ophthamol Vis Sci 18:288–302
Coxeter HSM (1973) Regular polytopes. Dover, New York
Fluorotron Master (1983) Manufacturer's instructions. Coherent Medical Division, Palo Alto, California
Herman R, Ohrloff C, Schalnus R (1985) Fluorophotometrie — eine empfindliche Technik zur quantitativen Messung der Hornhautendothelfunktion. Fortschr Ophthalmol 82:584–586
Jones RF, Maurice DM (1966) New methods of measuring the rate of aqueous flow in man with fluorescein. Exp Eye Res 5:208–211
Jumblatt MM, Matkin ED, Neufeld AH (1988) Pharmacological regulation of morphology and mitosis in cultured rabbit corneal endothelium. Invest Ophthamol Vis Sci 29:586–593
Ling T, Vannas A, Holden BA (1988) Long-term changes in corneal endothelial morphology following wounding in the cat. Invest Ophthalmol Vis Sci 29:1407–1412
Ludvigson MA, Sorenson RL (1980) Immunohistochemical localization of aldose reductase, rat eye and kidney. Diabetes 29:450–459
Matsuda M, Suda T, Manabe R (1984) Serial alterations in endothelial cell shape and pattern after intraocular surgery. Am J Ophthalmol 98:313–323
Matsuda M, Sawa M, Edelhauser HF, Bartels SP, Neufeld AH, Kenyon KR (1985) Cellular migration and morphology in corneal endothelial wound repair. Invest Ophthalmol Vis Sci 26:443–448
Maurice DM (1963) New objective fluorophotometer. Exp Eye Res 2:33–38
Maurice DM (1967) The use of fluorescein in ophthalmological research. Invest Ophthalmol 6:464–468
Maurice DM (1972) The location of the fluid pump in the cornea. J Physiol 221:43–44
Menerath JM, Coulangeon LM, Rigal D (1985) Fluorophotometric et endothelium corneen: resultats preliminaires, la clinique ophthalmologique. Edition des Laboratoires Martinet, Paris, pp 115–122
Mishima S, Trenberth S (1968) Permeability of the corneal endothelium to nonelectrolytes. Invest Ophthalmol 7:34–43
Ohrloff C (1987) Permeabilität der zellulären Grenzschichten der Hornhaut in vivo. Fortschr Ophthalmol 84:307–312
Ohrloff C, Schalnus R, Spitznas M (1986) Quantitative Kontrolle der Hornhautendothelfunktion durch Fluorophotometrie im vorderen Augensegment. Klin Monatsbl Augenheilkd 189:24–27
Olsen T (1979) Variations in endothelial morphology of normal corneas and after cataract extraction: a specular microscopic study. Acta Ophthamol 57:1014–1022
Ota Y, Mishima S, Maurice DM (1974) Endothelial permeability of the living cornea to fluorescein. Invest Ophthalmol 13:945–947
Sawa M, Araie M, Tanishima T (1983) A fluorophotometric study of the barrier functions in the anterior segment of the eye after intracapsular cataract extraction. Jpn J Ophthalmol 27:404–406
Sawa M, Sakanishi Y, Shimuzu H (1984) Fluorophotometric study of anterior segment barrier functions after extracapsular cataract extraction and posterior chamber intraocular lens implantation. Am J Ophthalmol 97:197–204
Tuft SJ, William KA, Coster DJ (1986) Endothelial repair in the rat cornea (abstr). Invest Ophthalmol Vis Sci 27:1199
Van Horn DL, Sendele DD, Seideman S, Buco PJ (1977) Regenerative capacity of the corneal endothelium in rabbit and cat. Invest Ophthalmol 16:597–608
Wilson SE, Bourne MW, O'Brien PC, Brubaker RF (1988) Endothelial functions and aqueous humor flow rate in patients with Fuchs' dystrophy. Am J Ophthalmol 106:270–278
Yablonski ME, Zimmermann T, Waltmann S, Becker B (1978) A fluorophotometric study of the effect of topical timolol on aqueous humor dynamics. Exp Eye Res 27:135–142
Yee RW, Geroski DH, Matsuda M, Champeau EJ, Meyer LA, Edelhauser HF (1985) Correlation of corneal endothelial pump site density, barrier function and morphology in wound healing. Invest Ophthalmol Vis Sci 26:1191–1193
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Goebbels, M., Spitznas, M. Endothelial barrier function after phacoemulsification: a comparison between diabetic and non-diabetic patients. Graefe's Arch Clin Exp Ophthalmol 229, 254–257 (1991). https://doi.org/10.1007/BF00167879
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00167879