Summary
The involvement of dopaminergic neurons of the hypothalamus in the modulation of histamine release was studied by the push-pull technique. The posterior hypothalamus of the conscious, freely moving rat was superfused with artificial cerebrospinal fluid (CSF) and the release of histamine was determined radioenzymatically in the superfusate. Agonists and antagonists of dopamine D1-, D2- and D3-receptors were dissolved in CSF and applied to the hypothalamus through the push-pull cannula.
Hypothalamic superfusion with the D1-, D2- and D3-receptor agonists dopamine or R(−)-apomorphine enhanced the release rate of histamine. (±) Apomorphine also enhanced the release of histamine, but to a lesser extent than did equimolar concentration of R(−)apomorphine. The D3-agonist quinpirole inhibited the release of histamine, while the D1-receptor agonist SKF 82958 [(±)-6-chloro-7,8-dihydroxy-3-allyl-l-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine] did not virtually influence the release of the neurotransmitter. On the other hand, [−]-sulpiride which predominantly blocks D2-receptors, decreased histamine release. Hypothalamic superfusion with SKF 83566 [(±)-7-bromo-8-hydroxy-3-methyl-l-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine], which seems to be a selective antagonist of D1-receptors, enhanced the release rate of histamine.
These findings suggest that dopaminergic neurons of the hypothalamus influence the release of histamine from its neurons in a dual way. D2-heteroreceptors stimulate the release of histamine, while D3-heteroreceptors seem to inhibit the release of this neurotransmitter. Both types of dopamine receptors might be located presynaptically on histaminergic neurons. Alternatively, D3- and D2-receptors might be located on histaminergic and non-histaminergic neurons, respectively.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arnt J, Hyttel J, Perregaard J (1987) D-1-Receptor agonists combinded with the selective D-2 agonist quinpirole facilitate the expression of oral stereotyped behaviour in rats. Eur J Pharmacol 133:137–145
Campbell A, Baldessarini RJ, Teicher MH, Neumeyer JL (1986) Behavioral effects of apomorphine isomers in the rat: selective locomotor-inhibitory effects of S(+)-N-n-propylnorapomorphine. Psychopharmacology 88:158–164
Gulat-Marnay C, Lafitte A, Arrang JM, Schwartz JC (1989) Regulation of histamine release and synthesis in the brain by muscarinic receptors. J Neurochem 52:248–254
Hess EJ, Creese I (1987) Biochemical characterization of dopamine receptors. In: Creese I, Fraser CM (eds) Dopamine receptors. Liss, New York, pp 1–27
Hill SH, Straw RM (1988) α2-adrenoceptor mediated inhibition of histamine release from rat cerebral cortical slices. Br J Pharmacol 95:1213 -1219
Khüver H, Barrera E (1953) A method for the combined staining of cells and fibers in the nervous system. J Neuropath Exp Neurol 12:400–403
Lehmann J, Smith RV, Langer SZ (1983) Stereoisomers of apomorphine differ in affinity and intrinsic activity at presynaptic dopamine receptors modulating [3H]dopamine and [3H]acetylcholine release in slices of cat caudate. Eur J Pharmacol 88:81–88
Murray AM, Waddington JL (1989) The induction of grooming and vacuous chewing by a series of selective D-1 dopamine receptor agonists: two directions of D-1: D-2 interaction. Eur J Pharmacol 160:377–384
O'Boyle KM, Waddington JL (1985) Identification of the enantiomers of SKF 83566 as specific and stereoselective antagonists at the striatal D-1 dopamine receptor: comparisons with the D-2 enantio-selectivity of Ro 22–1319. Eur J Pharmacol 106:219–220
Okakura K, Yamatodani A, Mochizuki T, Horii A, Wada H (1992) Glutamatergic regulation of histamine release from rat hypothalamus. Eur J Pharmacol 213:189–192
Palkovits M, Brownstein MJ (1989) Catecholamines in the central nervous system. In: Trendelenburg U, Weiner N (eds) Catecholamines II. Springer, Berlin Heidelberg New York, pp 1–161
Paxinos G, Watson C (1986) The rat brain in stereotaxic coordinates. Accademic Press, Sydney
Philippu A (1993) Modulation by heteroreceptors of the histamine release in the brain. In: Cacabelos R (ed) Basic and clinical neurosciences, Anuario Psiquiátrico, vol III. J R Prous, SA, Barcelona (in Press)
Prast H, Saxer A, Philippu A (1988) Pattern of in vivo release of endogenous histamine in the mamillary body and the amygdala. Naunyn-Schmiedeberg's Arch Pharmacol 337:53–57
Prast H, Heistracher M, Philippu A (1991) In vivo modulation of the histamine release in the hypothalamus by adrenoceptor agonists and antagonist. Naunyn-Schmiedeberg's Arch Pharmacol 344:183–186
Sokoloff P, Giros B, Martres MP, Bouthenet ML, Schwartz JC (1990) Molecular cloning and characterization of a novel dopamine receptor (D3) as a target for neuroleptics. Nature 347:146–151
Author information
Authors and Affiliations
Additional information
This work was supported by the Fonds zur Förderung der wissenschaftlichen Forschung
Correspondence to H. Prast at the above address
Rights and permissions
About this article
Cite this article
Prast, H., Heistracher, M. & Philippu, A. Modulation by dopamine receptors of the histamine release in the rat hypothalamus. Naunyn-Schmiedeberg's Arch Pharmacol 347, 301–305 (1993). https://doi.org/10.1007/BF00167449
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00167449