Abstract
An Igh-V library was constructed from the head kidney cytoplasmic RNA of an 8.5-month-old non-immunized rainbow trout, Oncorhynchus mykiss, using the 5′ RACE polymerase chain reaction. Six new Igh-V segments were identified, bringing to nine the number of Igh-V families actually defined in that species. A phylogenetic analysis shows that these nine Igh-V families can be classified into three major groups. The first includes the Igh-V1, Igh-V3, Igh-V4, and Igh-V7 families, and is homologous to the human and mouse Group III Igh-V families. The second includes the Igh-V5, Igh-V8, and Igh-V9 families and is more closely related to the Group I and Group II human and mouse Igh-V families. The third group includes the Igh-V2 and Igh-V6 families, which are not closely related to any other vertebrate Igh-V gene. Six Igh-J segments were characterized. They can recombine with Igh-V segments belonging to different families and there is a high level of junctional diversity between the Igh-V and Igh-J segments.
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The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers L28741 (Igh-V4), L28742 (Igh-V5.1), L28744 (Igh-V6), L28745 (Igh-V7), L28746 (Igh-V8), L28747 (Igh-V9), and L28805 (Igh-V5.2)
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Roman, T., Charlemagne, J. The immunoglobulin repertoire of the rainbow trout (Oncorhynchus mykiss): definition of nine Igh-V families. Immunogenetics 40, 210–216 (1994). https://doi.org/10.1007/BF00167081
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DOI: https://doi.org/10.1007/BF00167081