Abstract
The formation of anaerobic granular sludge on wastewater from sugar-beet processing was examined in upflow anaerobic sludge blanket reactors. Two strategies were investigated: addition of high-energy substrate, i.e. sugars, and varying the reactor liquid surface tension. When there were insufficient amounts of sugars i.e. less than 7% of the chemical O2 demand of the influent, no granulation was observed; moreover lowering the reactor liquid surface tension below 48 mN/m was found to increase biomass wash-out. On the other hand, when there were sufficient sugars, granular sludge growth occurred; moreover operating the reactor at a low reactor liquid surface tension reduced biomass wash-out and increased granular yield.
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APHA (1989) Standard methods for the examination of water and wastewater. American Public Health Association, Washington DC
Bremner JM, Keeney RD (1965) Steam distillation methods for determination of ammonium, nitrate and nitrite. Anal Chim Acta 32: 485–495
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chim Acta 28: 350–356
Grotenhuis JTC, Kissel JC, Plugge CM, Stams AJM, Zehnder AJB (1991) Role of substrate concentration in particle size distribution of methanogenic granular sludge in UASB reactor. Water Res 25: 21–27
Holdeman LV, Cato EP Moore WEC (1977) Anaerobic laboratory manual. Virginia polytechnic institute and state university Blacksberg, Virginia, pp 130
Hulshoff Pol LW, Lettinga G (1986) New technologies for anaerobic wastewater treatment. Water Sci Technol 18: 41–53
Lettinga G, Hulshoff Pol LW (1991) UASB-process design for various types of wastewaters. Water Sci Technol 24: 87–107
Lettinga, G, van Velsen AFM, Hobma SM, de Zeeuw W, Klapwijk A (1980) Use of the upflow sludge blanket (USB) reactor concept for biological wastewater treatment. Biotechnol Bioeng 22: 699–734
Lettinga G, de Zeeuw W, Hulshoff Pol L, Wiegant W, Rinzema A (1985) Anaerobic wastewater treatment based on biomass retention with emphasis on the UASB-process. In: China State Biogas Association (eds) Proceedings of the Fourth International Symposium on Anaerobic Digestion, Guangzhou, China. The China State Association, Beijing, China, pp 279–302
MacLeod FA, Guiot SR, Costerton JW (1990) Layered structure of bacterial aggregates produced in an upflow anaerobic sludge bed and filter reactor. Appl Environ Microbiol 56: 1598–1607
Merck E (1973) Die Untersuchung von Wasser. Darmstadt, pp 115
Morgan JW, Evison LM, Forster CF (1991) The impact of anaerobic extracellular polymers on the metabolism of granular anaerobic sludge. Environ Technol 12: 421–430
Ross WR (1984) The phenomenon of sludge pelletisation in the anaerobic treatment of a maize processing waste. Water SA (Pretoria) 10: 197–204
Shelton DR, Tiedje JM (1984) General method for determining anaerobic biodegradation potential. Appl Environ Microbiol 47: 850–857
Thaveesri J, Daffonchio D, Liessens B, Van der Meeren P, Verstraete W (1995) Granulation and sludge bed stability in UASB reactors in relation to surface thermodynamics. Appl Environ Microbiol (in press).
Uemura S, Harada H (1993) Microbial characteristics of methanogenic sludge consortia developed in thermophilic UASB reactors. Appl Microbiol Biotechnol 39: 654–660
Valcke D, Verstraete W (1983) A practical method to estimate the acetoclastic methanogenic biomass in anaerobic sludge. J Water Pollut Control Fed 52: 720–729
Vanderhaegen B, Ysebaert B, Favere K, Van Wambeke M, Peeters T, Panic V, Vandenlangenbergh V, Verstraete W (1992) Acidogenesis in relation to in-reactor granule yield. Water Sci Technol 25: 21–30
Van Wambeke M, Grusenmeyer S, Verstraete W, Longry R (1990) Sludge bed growth in an UASB reactor treating potato processing wastewater. Process Biochem 25:181–186
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Thaveesri, J., Liessens, B. & Verstraete, W. Granular sludge growth under different reactor liquid surface tensions in lab-scale upflow anaerobic sludge blanket reactors treating wastewater from sugar-beet processing. Appl Microbiol Biotechnol 43, 1122–1127 (1995). https://doi.org/10.1007/BF00166936
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DOI: https://doi.org/10.1007/BF00166936