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Granular sludge growth under different reactor liquid surface tensions in lab-scale upflow anaerobic sludge blanket reactors treating wastewater from sugar-beet processing

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Abstract

The formation of anaerobic granular sludge on wastewater from sugar-beet processing was examined in upflow anaerobic sludge blanket reactors. Two strategies were investigated: addition of high-energy substrate, i.e. sugars, and varying the reactor liquid surface tension. When there were insufficient amounts of sugars i.e. less than 7% of the chemical O2 demand of the influent, no granulation was observed; moreover lowering the reactor liquid surface tension below 48 mN/m was found to increase biomass wash-out. On the other hand, when there were sufficient sugars, granular sludge growth occurred; moreover operating the reactor at a low reactor liquid surface tension reduced biomass wash-out and increased granular yield.

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References

  • APHA (1989) Standard methods for the examination of water and wastewater. American Public Health Association, Washington DC

    Google Scholar 

  • Bremner JM, Keeney RD (1965) Steam distillation methods for determination of ammonium, nitrate and nitrite. Anal Chim Acta 32: 485–495

    Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chim Acta 28: 350–356

    Google Scholar 

  • Grotenhuis JTC, Kissel JC, Plugge CM, Stams AJM, Zehnder AJB (1991) Role of substrate concentration in particle size distribution of methanogenic granular sludge in UASB reactor. Water Res 25: 21–27

    Google Scholar 

  • Holdeman LV, Cato EP Moore WEC (1977) Anaerobic laboratory manual. Virginia polytechnic institute and state university Blacksberg, Virginia, pp 130

    Google Scholar 

  • Hulshoff Pol LW, Lettinga G (1986) New technologies for anaerobic wastewater treatment. Water Sci Technol 18: 41–53

    Google Scholar 

  • Lettinga G, Hulshoff Pol LW (1991) UASB-process design for various types of wastewaters. Water Sci Technol 24: 87–107

    Google Scholar 

  • Lettinga, G, van Velsen AFM, Hobma SM, de Zeeuw W, Klapwijk A (1980) Use of the upflow sludge blanket (USB) reactor concept for biological wastewater treatment. Biotechnol Bioeng 22: 699–734

    Google Scholar 

  • Lettinga G, de Zeeuw W, Hulshoff Pol L, Wiegant W, Rinzema A (1985) Anaerobic wastewater treatment based on biomass retention with emphasis on the UASB-process. In: China State Biogas Association (eds) Proceedings of the Fourth International Symposium on Anaerobic Digestion, Guangzhou, China. The China State Association, Beijing, China, pp 279–302

    Google Scholar 

  • MacLeod FA, Guiot SR, Costerton JW (1990) Layered structure of bacterial aggregates produced in an upflow anaerobic sludge bed and filter reactor. Appl Environ Microbiol 56: 1598–1607

    Google Scholar 

  • Merck E (1973) Die Untersuchung von Wasser. Darmstadt, pp 115

  • Morgan JW, Evison LM, Forster CF (1991) The impact of anaerobic extracellular polymers on the metabolism of granular anaerobic sludge. Environ Technol 12: 421–430

    Google Scholar 

  • Ross WR (1984) The phenomenon of sludge pelletisation in the anaerobic treatment of a maize processing waste. Water SA (Pretoria) 10: 197–204

    Google Scholar 

  • Shelton DR, Tiedje JM (1984) General method for determining anaerobic biodegradation potential. Appl Environ Microbiol 47: 850–857

    Google Scholar 

  • Thaveesri J, Daffonchio D, Liessens B, Van der Meeren P, Verstraete W (1995) Granulation and sludge bed stability in UASB reactors in relation to surface thermodynamics. Appl Environ Microbiol (in press).

  • Uemura S, Harada H (1993) Microbial characteristics of methanogenic sludge consortia developed in thermophilic UASB reactors. Appl Microbiol Biotechnol 39: 654–660

    Google Scholar 

  • Valcke D, Verstraete W (1983) A practical method to estimate the acetoclastic methanogenic biomass in anaerobic sludge. J Water Pollut Control Fed 52: 720–729

    Google Scholar 

  • Vanderhaegen B, Ysebaert B, Favere K, Van Wambeke M, Peeters T, Panic V, Vandenlangenbergh V, Verstraete W (1992) Acidogenesis in relation to in-reactor granule yield. Water Sci Technol 25: 21–30

    Google Scholar 

  • Van Wambeke M, Grusenmeyer S, Verstraete W, Longry R (1990) Sludge bed growth in an UASB reactor treating potato processing wastewater. Process Biochem 25:181–186

    Google Scholar 

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Thaveesri, J., Liessens, B. & Verstraete, W. Granular sludge growth under different reactor liquid surface tensions in lab-scale upflow anaerobic sludge blanket reactors treating wastewater from sugar-beet processing. Appl Microbiol Biotechnol 43, 1122–1127 (1995). https://doi.org/10.1007/BF00166936

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  • DOI: https://doi.org/10.1007/BF00166936

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