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Energetics and product formation by Saccharomyces cerevisiae grown in anaerobic chemostats under nitrogen limitation

Applied Microbiology and Biotechnology volume 43, pages 1034–1038 (1995)Cite this article

Abstract

Anaerobic fermentation of glucose (20 g/l) by Saccharomyces cerevisiae CBS 8066 was studied in a chemostat (dilution rate = 0.05−0.25 h−1) at different concentrations of the nitrogen source (5.00 g/l or 0.36 g/l ammonium sulphate). The ethanol yield (g ethanol produced/g glucose consumed) was found to be higher and the glycerol yield (g glycerol formed/g glucose consumed) lower during nitrogen limitation than under carbon limitation. The biomass yield on ATP (g dry weight biomass produced/mol ATP consumed) was consequently found to be lower during nitrogen-limited conditions.

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References

  • Bauchop T, Elsden SR (1960) The growth of micro-organisms in relation to their energy supply. J Gen Microbiol 23:457–469

    Google Scholar 

  • Blomberg A, Adler L (1992) Physiology of osmotolerance in fungi. Adv Microbiol Physiol 33:145–212

    Google Scholar 

  • Bruinenberg PM, Dijken JP van, Scheffers WA (1983) A theoretical analysis of NADPH production and consumption in yeasts. J Gen Microbiol 129:953–964

    Google Scholar 

  • Fiechter A, Fuhrmann F, Käppeli O (1981) Regulation of glucose metabolism in growing yeast cells. Adv Microbiol Physiol 22:123–183

    Google Scholar 

  • Lagunas R, Gancedo JM (1973) Reduced pyridine-nucleotides balance in glucose-growing Saccharomyces cerevisiae. Eur J Biochem 37:90–94

    Google Scholar 

  • Lagunas R, Ruiz E (1988) Balance and consumption of ATP in ammonium-starved Saccharomyces cerevisiae. J Gen Microbiol 134:2507–2511

    Google Scholar 

  • Larsson C, Stockar U von, Marison I, Gustafsson L (1993) Growth and metabolism of Saccharomyces cerevisiae in chemostat cultures under carbon-, nitrogen-, or carbon- and nitrogen- limiting conditions. J Bacteriol 175:4809–4816

    Google Scholar 

  • Lidén G, Jacobsson V, Niklasson C (1993) The effect of carbon dioxide on xylose fermentation by Pichia stipitis. Appl Biochem Biotechnol 38:27–40

    Google Scholar 

  • Neijssel OM, Tempest DW (1987) Relationships between nutrient status of the environment and metabolic fluxes in microorganisms. Dechema Monogr 105:29–42

    Google Scholar 

  • Neijssel OM, Tempest DW (1990) The role of futile cycles in the energetics of bacterial growth. Biochim Biophys Acta 1018:252–255

    Google Scholar 

  • Nilsson A, Larsson C, Gustafsson L (1995) Catabolic capacity of Saccharomyces cerevisiae in relation to the physiological state and maintenance requirement. Thermochim Acta (in press)

  • Oelz R, Larsson K, Adler L, Gustafsson L (1993) Energy flux and osmoregulation of Saccharomyces cerevisiae grown in chemostats under NaCl stress. J Bacteriol 175:2205–2213

    Google Scholar 

  • Oura E (1977) Reaction products of yeast fermentations. Proc Biochem 12:19–21, 35

    Google Scholar 

  • Schatzmann H (1975) Anaerobes Wachstum von Saccharomyces cerevisiae. Regulatorische Aspekte des glycolytischen und respirativen Stoffwechsels. Diss. 5504, ETH, Zurich, Zwitzerland

    Google Scholar 

  • Stouthamer AH (1979) The search for correlation between theoretical and experimental growth yields. Int Rev Biochem 21:1–47

    Google Scholar 

  • Van Urk H, Mak PR, Scheffers WA, Dijken JP van (1988) Metabolic responses of Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621 upon transition from glucose limitation to glucose excess. Yeast 4:283–291

    Google Scholar 

  • Verduyn C, Postma E, Scheffers WA, van Dijken JP (1990) Physiology of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat culture. J Gen Microbiol 136:395–403

    Google Scholar 

  • Visser W, Scheffers WA, Batenburg-van der Vegte WH, Dijken JP van (1990) Oxygen requirements of yeasts. Appl Environ Microbiol 56:3785–3792

    Google Scholar 

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Affiliations

  1. Department of Chemical Reaction Engineering, Chalmers University of Technology, S-412 96, Göteborg, Sweden

    G. Lidén, A. Persson & C. Niklasson

  2. Department of General Marine Microbiology, University of Göteborg, Medicinaregat. 9C, S-413 90, Göteborg, Sweden

    L. Gustafsson

Authors
  1. G. Lidén
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  2. A. Persson
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  3. C. Niklasson
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  4. L. Gustafsson
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Lidén, G., Persson, A., Niklasson, C. et al. Energetics and product formation by Saccharomyces cerevisiae grown in anaerobic chemostats under nitrogen limitation. Appl Microbiol Biotechnol 43, 1034–1038 (1995). https://doi.org/10.1007/BF00166921

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  • DOI: https://doi.org/10.1007/BF00166921

Keywords

  • Glucose
  • Biomass
  • Fermentation
  • Glycerol
  • Saccharomyces Cerevisiae