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Restriction site map of the Chlorella virus PBCV-1 genome

Plant Molecular Biology volume 9, pages 247–257 (1987)Cite this article

Abstract

The virus PBCV-1, which replicates in a Chlorella-like green alga, has a dsDNA genome. The DNA was mapped for BamHI, HindIII, and PstI restriction sites. The resulting map has a size of 333 kbp and is circular—indicating either covalently closed circular DNA or circularly permuted linear DNA. Several regions of repetitive DNA were also identified and located on the restriction map.

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References

  1. Bolivar F, Backman K: Plasmids of Escherichia coli as cloning vectors. In: Wu R (ed.) Methods in Enzymology, Vol. 68, pp 245–267 (1979).

  2. Case ST: Selective deletion of large segments of Balbiani ring DNA during molecular cloning. Gene 20: 169–176 (1982).

    Google Scholar 

  3. Darai G, Delius H, Clarke J, Apfel H, Schnitzler P, Flugel RM: Molecular cloning and physical mapping of the genome of fish lymphocystis disease virus. Virol 146: 292–301 (1985).

    Google Scholar 

  4. Delius H, Darai G, Flugel RM: DNA analysis of insect iridescent virus 6: Evidence for circular permutation and terminal redundancy. J Virol 49: 609–614 (1984).

    Google Scholar 

  5. Girton LE: Organization of the dsDNA genome of the Chlorella algal virus PBCV-1. Ph.D. Dissertation. University of Nebraska (1986).

  6. Goorha R, Murti K: The genome of frog virus 3, an animal DNA virus, is circularly permuted and terminally redundant. Proc Natl Acad Sci, USA 79: 248–252 (1982).

    Google Scholar 

  7. Holmes DS, Quigley M: A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114: 193–197 (1981).

    Google Scholar 

  8. Leach DRF, Stahl FW: Viability of lambda phages carrying a perfect palindrome in absence of recombination nucleases. Nature 305: 448–451 (1983).

    Google Scholar 

  9. Lee MH, Willis DB: Restriction endonuclease mapping of the frog virus 3 genome. Virol 126: 317–327 (1983).

    Google Scholar 

  10. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1982).

    Google Scholar 

  11. Meints RH, Schuster AM, VanEtten JL: Chlorella viruses. Plant Mol Biol Rept 3: 180–187 (1985).

    Google Scholar 

  12. Murray NE, Brammer WJ, Murray K: Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet 150: 53–61 (1977).

    Google Scholar 

  13. Raleigh EA, Wilson G: Escherichia coli K-12 restricts DNA containing 5-methylcytosine. Proc Natl Acad Sci, USA 83: 9070–9074 (1986).

    Google Scholar 

  14. Schuster AM, Girton L, Burbank DE, VanEtten JL: Infection of a Chlorella-like alga with the virus PBCV-1: Transcriptional studies. Virol 148: 181–189 (1986).

    Google Scholar 

  15. Skrdla MP, Burbank DE, Xia Y, Meints RH, VanEtten JL: Structural proteins and lipids in a virus, PBCV-1, which replicates in a Chlorella-like alga. Virol 135: 308–315 (1984).

    Google Scholar 

  16. VanEtten JL, Meints RH, Kuczmarski D, Burbank DE, Lee K: Viruses of symbiotic Chlorella-like algae isolated from Paramecium bursaria and Hydra viridis. Proc Natl Acad Sci, USA 79: 3867–3871 (1982).

    Google Scholar 

  17. VanEtten JL, Burbank DE, Kuczmarski D, Meints RH: Virus infection of culturable Chlorella-like algae and development of a plaque assay. Science 219: 994–996 (1983).

    Google Scholar 

  18. VanEtten JL, Burbank DE, Xia Y, Meints RH: Growth cycle of a virus, PBCV-1, that infects Chlorella-like algae. Virol 126: 117–125 (1983).

    Google Scholar 

  19. VanEtten JL, Burbank DE, Joshi J, Meints RH: DNA synthesis in a Chlorella-like alga following infection with the virus, PBCV-1. Virol 134: 443–449 (1984).

    Google Scholar 

  20. VanEtten JL, Schuster AM, Girton L, Burbank DE, Swinton D, Hattman S: DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga. Nucleic Acids Res 13: 3471–3478 (1985).

    Google Scholar 

  21. VanEtten JL, Xia Y, Meints RH: Viruses of a Chlorella-like green alga. In: Kosuge T, Nester EW (eds) Plant-Microbe Interactions, Vol. II. Macmillan Publishing Co., New York (1987) pp 307–325.

    Google Scholar 

  22. Wahl GM, Stern M, Stark GR: Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl paper and rapid hybridization using dextran sulfate. Proc Natl Acad Sci, USA 76: 3683–3687 (1979).

    Google Scholar 

  23. Xia Y, Burbank DE, Uher L, Rabussay D, VanEtten JL: Restriction endonuclease activity induced by PBCV-1 virus infection of a Chlorella-like green alga. Mol Cell Biol 6: 1430–1439 (1986).

    Google Scholar 

  24. Xia Y, VanEtten JL: DNA methyltransferase induced by PBCV-1 virus infection of a Chlorella-like green alga. Mol Cell Biol 6: 1440–1445 (1986).

    Google Scholar 

  25. Yanisch-Perron C, Vieira J, Messing J: Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103–119 (1985).

    Google Scholar 

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Author notes

  1. Lois E. Girton

    Present address: Department of Botany, Iowa State University, 50011, Ames, IA, USA

Affiliations

  1. Department of Plant Pathology, University of Nebraska, 68583-0722, Lincoln, NE, USA

    Lois E. Girton & James L. van Etten

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  1. Lois E. Girton
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  2. James L. van Etten
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Girton, L.E., van Etten, J.L. Restriction site map of the Chlorella virus PBCV-1 genome. Plant Mol Biol 9, 247–257 (1987). https://doi.org/10.1007/BF00166461

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  • DOI: https://doi.org/10.1007/BF00166461

Key words

  • Chlorella virus dsDNA genome
  • restriction map of PBCV-1 genome