Abstract
An analysis by restriction endonuclease digestion of ribosomal DNA (rDNA) was carried out in natural populations of Apodemus speciosus, a field mouse that is endemic to Japan. Two restriction sites, the EcoRI (E3) and DraI (D4) sites, in the nontranscribed spacer region downstream from the gene for 28S RNA showed polymorphism within and between individuals in the populations from the Japanese main islands. By contrast, populations from the small adjoining islands which are thought to have separated from the main islands 1–2 × 104 years ago showed relatively low levels of polymorphism within and between individuals, i.e., one of the polymorphic bands in the case of each enzyme was predominant in these populations, irrespective of the variants. These results indicate that the rate of fixation of site variations depends on population size and that the direction of fixation is random. Furthermore, each polymorphic restriction site seems to be fixed independently.
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References
Arnheim N (1983) Concerted evolution of multigene families. In: Nei M, Koehn RK (eds) Evolution of genes and proteins. Sinauer, Sunderland, MA, pp 38–61
Arnheim N, Krystal M, Schmickel R, Wilson G, Ryder O, Zimmer E (1980) Molecular evidence for genetic exchanges among ribosomal genes on nonhomologous chromosomes in man and apes. Proc Natl Acad Sci USA 77:7323–7327
Corbet GB (1978) The mammals of the palaearctic region: a taxonomic review. Cornell University Press, Ithaca
Dover G (1982) Molecular drive: a cohesive mode of species evolution. Nature 299:111–117
Imaizumi Y (1962) On the species formation of the Apodemus speciosus group, with special reference of the importance of relative values in classification, part 1. Bull Nat Sci Mus Tokyo 5:163–259
Imaizumi Y (1964) On the species formation of the Apodemus speciosus group, with special reference of the importance of relative values in classification, part 2. Bull Nat Sci Mus Tokyo 7:127–177
Kominami R, Urano Y, Mishima Y, Muramatsu M (1981) Organization of ribosomal RNA gene repeats of the mouse. Nucleic Acids Res 9:3219–3233
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Matsuo Y and Yamazaki T (1989) Nucleotide variation and divergence in the histone multigene family in Drosophila melanogaster. Genetics 122:87–97
Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
Nevo E, Beiles A (1988) Ribosomal DNA nontranscribed spacer polymorphism in subterranean mole rats: genetic differentiation, environmental correlates, and phylogenetic relationships. Evol Ecol 2:139–156
Ohta T (1980) Evolution and variation of multigene families. Springer-Verlag, New York
Saitoh M, Obara Y (1986) Chromosome banding patterns in five intraspecific taxa of the large Japanese field mouse, Apodemus speciosus. Zool Sci 3:785–792
Smith GP (1976) Evolution of repeated DNA sequences by unequal crossover. Science 191:528–535
Seperack P, Slatkin M, Arnheim N (1988) Linkage disequilibrium in human ribosomal genes: implication for multigene family evolution. Genetics 119:943–949
Suzuki H, Tsuchiya K, Sakaizumi M, Wakana S, Gotoh O, Saitou N, Moriwaki K, Sakurai S (1990) Differentiation of restriction sites in ribosomal DNA in the genus Apodemus. Biochem Genet 28:137–148
Tsuchiya K, Moriwaki K, Yosida TH (1973) Cytogenetical survey in wild populations of Japanese wood mouse, Apodemus speciosus and its breeding. Exp Anim 22:221–229
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Correspondence to: H. Suzuki
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Suzuki, H., Tsuchiya, K., Sakaizumi, M. et al. Evolution of restriction sites of ribosomal DNA in natural populations of the field mouse, Apodemus speciosus . J Mol Evol 38, 107–112 (1994). https://doi.org/10.1007/BF00166157
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DOI: https://doi.org/10.1007/BF00166157