Abstract
The goal of this work was to select higher fungi for the production of enzymes able to produce plant protoplasts. The hydrolytic capacity of various species of wood-detsroying fungi was examined to select those with the maximal hydrolytic capacity. The selection criteria included attack on cellulose, and oligo-and polysaccharides by each fungus species. Lenzites elegans, gonoderma applanatum and Pycnoporus sanguineus produced thr richest enzymatic mixtures, which were evaluated for their protoplasting ability. L. elegans has the best enzymatic mixture able to [roduced plant protoplasts with the shortest time for maceration of plant tissues, followed by G. applanatum and P. sanguineus. The potential of these fungi and the simplicity of enzyme proudtcion suggest the possibility of commercial applications.
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Correepondence to: A. R. Sampietro
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Leal, D.P., Isla, M.I., Vattuone, M.A. et al. Production of plant protoplasts by enzymes from selected wood-destroying fungi. Appl Microbiol Biotechnol 41, 58–61 (1994). https://doi.org/10.1007/BF00166082
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DOI: https://doi.org/10.1007/BF00166082