Abstract
Lens capsules of patients of advanced age, obtained after extracapsular cataract surgery, were carefully prepared for a combined LM, TEM and SEM investigation, after preliminary washing and mounting onto a holder in a buffer solution. After pre-fixation with GA, samples were postfixed for LM/TEM and OsO4/K4Fe(CN)6 and stained with toluidineblue/basic fuchsine for LM and with uranyl acetate/lead citrate for TEM; for SEM the GA-pre-fixed samples were post-fixed by the Tannine Arginine-OsO4 non-coating technique. At LM-level discrimination between healthy and degenerating cells was possible after toluidine staining. At SEM-level protrusion of the cell nucleus and fibrillation and blebbing of the cell membrane as the result of capsular degeneration could be observed with the TAO-method. At TEM-level protrusion of the cell nucleus, degeneration of the cytoplasm, ballooning of the mitochondria, the presence of microfilaments and the occurrence of vacuoles were visible as the result of capsular degeneration on cataract formation.
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Bahr GF. Elektronenmikroskopische Untersuchungen uber den lamellaren Aufbau der Linsenkapsel des Auges. Von Graefes Arch Ophth 1954; 155: 635–638.
Beckmann H, Khodadayan C, Schnoy N. Licht- und Elektronenmikroskopie der humanen, anterioren kataraktkapsel. Forschr Ophthalmol 1989; 86: 556–560.
Blaauw EH, Jonkman MF, Gerrits PO. A rapid connective tissue stain for glycol methacrylate embedded tissue. Acta Morphol Neerl-Scand 1987; 25: 167–172.
Jongebloed WL, Kalicharan D, Havinga P. Experiences with non-coating techniques like OTOTO and TAO for biological tissues in the SEM. Ultramicrosc 1982; 9: 422.
Kalicharan D, Dijk F, Jongebloed WL. A comparison of standard TEM and non-coating SEM preparation procedures on intestine material. Ultramicrosc 1984; 14: 174.
Karim AKA. The human anterior lens capsule: Cell density, morphology and mitotic index in normal and cataractous lenses. Exp Eye Res 1987; 45: 865–874.
Murphy JA. Non-coating techniques to render biological specimens conductive. Scann. Electr Microsc 1978; 2: 175–195.
Chaplin AJ. Tannic acid in histology: An historical perspective. Stain Technology 1985; 60: 219–231.
Eshaghian J, Streeten BM. Human posterior subcapsular cataract: An Ultrastyructural study of the posteriorly migrating cells. Arch Ophthalmol 1980; 98: 134–143.
Font RL, Brownstein S. A light and electronmicroscopic study of anterior subcapsular cataracts. Am J Ophthalmol 1974; 78: 972–984.
Pau H, Nowotny GEK. Ultrastructural investigations on anterior capsular cataract: Cellular elements and their relationship to basement membrane and collagen synthesis. Graefes Arch Klin Exp Ophthalmol 1985; 223: 41–46.
Salman L von. The lens epithelium in the pathogenesis of cataract. The 13th Edward Jackson memoral lecture. Am J Ophthalmol 1957; 44: 159–169.
Bruijn de WC. A modified OsO4-(double) fixation procedure with selective glycogen contrast. In: Bocciarelli DS, ed. Electron microscopy, Vol. 2 65–66. Roma: Tipografia Poliglotta Vaticana, 1968: 61–66.
Los LI, Jongebloed WL, Worst JGF. Lens-capsule material of human and animal origin, studied by SEM. Doc Ophthalmol 1989; 72: 357–365.
Jongebloed WL, Los LI, Worst JGF. Morphology of donor lens-capsule material, studied by SEM. Doc Ophthalmol 1990; 75: 343–350.
Kalicharan D, Jongebloed WL. TEM examination of four different tissues prepared for SEM either by OTOTO, GOTO or GOTU. Ultramicrosc 1988; 24: 438.
Karnovsky MJ. Use of ferrocyanide-reduced osmium tetroxide in electronmicroscopy. J Cell Biol 1971; 51: 146A.
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Jongebloed, W.L., Kalicharan, D., Los, L.I. et al. Morphological aspects of human lens capsules. Doc Ophthalmol 78, 317–324 (1991). https://doi.org/10.1007/BF00165695
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DOI: https://doi.org/10.1007/BF00165695