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The effect of denbufylline on the viscosity of rat whole blood and on the deformability (filterability) of rat blood cell suspensions

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Summary

The novel alkylxanthine, denbufylline [1,3-di-n-butyl-7-(2-oxopropyl)-xanthine] has been examined, in vitro, for effects on the viscosity of rat whole blood and on the filterability of rat blood cell suspensions. For comparison, pentoxifylline was also examined for theological activity. Denbufylline reduced the viscosity of whole blood at all shear rates utilised, up to 128.5 s−1. The effect was, however, more pronounced at a low (0.7 s−1) than at a high (94.5 s−1) shear rate indicating that the compound reduces blood cell aggregation and increases blood cell deformability. Denbufylline also increased the filterability of blood cell suspensions. This is further evidence that the compound increases the deformability of blood cells. Denbufylline elevated the filterability of both pure erythrocyte and mixed erythrocyte/leucocyte suspensions, the effect being greatest with the latter. This suggests that denbufylline may influence the deformability of both red and white blood cells. However, the effect on white blood cells, under the experimental conditions employed, is apparently more marked. Pentoxifylline also reduced the viscosity of rat whole blood and increased the filterability of rat blood cell suspensions. However, denbufylline was 10–100-fold more potent in these tests than pentoxifylline.

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Authors and Affiliations

  1. Beecham-Wülfing Research Laboratories, D-3212, Gronau (Leine), Federal Republic of Germany

    J. J. Jukna & C. D. Nicholson

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  1. J. J. Jukna
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  2. C. D. Nicholson
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Part of this work was presented at the 1985 Aachen Joint Meeting of the Belgian, Dutch and German Pharmacological and Toxicological Societies

Send offprint requests to C. D. Nicholson at the above address

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Jukna, J.J., Nicholson, C.D. The effect of denbufylline on the viscosity of rat whole blood and on the deformability (filterability) of rat blood cell suspensions. Naunyn-Schmiedeberg's Arch Pharmacol 335, 445–448 (1987). https://doi.org/10.1007/BF00165561

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