Abstract
We describe a technique by which large numbers of peritoneal macrophages can be harvested without harming the experimental animal. The macrophages can be reintroduced into the same animal avoiding possible immune reactions associated with injection of foreign cells or other antigenic materials.
In an attempt to obstruct aqueous outflow rabbit peritoneal macrophages engorged with mineral oil were injected intracamerally in the same rabbit's eye. The number of cells per single injection varied from 4.0 × 104 to 1.4 × 106 in eight rabbits. In three animals daily injections of 1 × 106 cells were performed. Using the Perkins applanation tonometer daily and hourly, no pressure elevation could be detected.
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Feder, R.S., Dueker, D.K. Can macrophages cause obstruction to aqueous outflow in rabbits?. Int Ophthalmol 7, 87–93 (1984). https://doi.org/10.1007/BF00165109
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DOI: https://doi.org/10.1007/BF00165109