Skip to main content
Log in

Morphology of donor lens-capsule material studied by SEM

  • Published:
Documenta Ophthalmologica Aims and scope Submit manuscript

Abstract

The lens capsule of a 70-year-old male donor with a cataractous lens was carefully prepared for SEM by first washing the capsule with buffer solution to remove lens-fibres and subsequently attaching it to silicon rubber. During the fixation and drying stages of the preparation procedure the capsule stayed attached to the rubber substratum. In the equatorial zone germinating cells were found with knob-shaped microvilli, closely connected to lensfibres. Large units of pathological capsule epithelial cells were found, only slightly inter connected by a few pseudoposia. In addition, single pathological epithelial cells with pseudopodia, arranged on top of the cell in a rosette-like configuration, were found at certain locations. Both types are probably related to the original lens-cataract.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Figueras MJ, Jongebloed WL, Worst JGF. Scanning electron-microscopic study of eye tissue. Am Intra-Ocular Impl Soc J 1984; 10: 169–175.

    Google Scholar 

  2. Jongebloed WL, Rijneveld WJ, Cuperus PL, van Andel P, Worst JGF. Stainless steel as suturing material in human corneas. A SEM-study Doc Ophthalmol 1988; 70: 154–154.

    Google Scholar 

  3. Jongebloed WL, Kalicharan D, Havinga P. Experiences with non-coating techniques like OTOTO and TAO for biological tissues in the SEM. Ultramicrosc 1982; 9: 422.

    Google Scholar 

  4. Kalicharan D, Dijk F, Jongebloed WL. A comparison of standard TEM and non-coating SEM preparation procedures on intestine material. Ultramicrosc 1984; 4: 411.

    Google Scholar 

  5. Kalicharan D, Jongebloed WL. TEM of CPD and FD-samples of rat intestine, prepared according OTOTO, GOTO and GOTU respectively. Ultramicrosc 1986; 2: 99–100.

    Google Scholar 

  6. Kappelhof JP. The fate of retained lens material and intraocular lenses. In: Extracapsular Lens Extraction 1987: 47–55.

  7. Los LI, Jongebloed WL, Worst JGF. Lens-capsule material of human origin, studied by SEM. Soc Opthalmol 1989; 72: 357–365

    Google Scholar 

  8. Versura M, Maltarello MC. The role of scanning electron-microscopy in ophthalmic science. Scanning Microscopy 1988; 2(3): 1695–1723.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jongebloed, W.L., Los, L.I., Dijk, F. et al. Morphology of donor lens-capsule material studied by SEM. Doc Ophthalmol 75, 343–350 (1990). https://doi.org/10.1007/BF00164849

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00164849

Key words

Navigation