Summary
The pH decrease in a phosphate buffer due to fermentation of glucose to lactic acid by non-growing Lactobacillus plantarum cells has been studied. The method used offers a quick and reproducible way of measuring the glucose-fermenting activity of L. plantarum. The maximum observed velocity of pH decrease is linear with the biomass concentration and is defined as the activity of the cell suspension. With L. plantarum, recalculation of this arbitrary unit (ΔpH·min−1 per gram dry weight) to a conceivable unit of lactic acid production rate (mol·min−1 per gram dry weight) is possible. This recalculation is based on the titration theory of a weak base with a weak acid. The same theory together with the lactic acid production kinetics of L. plantarum is applied to model the entire pH-time curve.
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References
Alberty RA, Smith RM, Bock RM (1951) The apparent ionization constants of the adenosinephosphates and related compounds. J Biol Chem 193:425–434
Anderson EB, Meanwell LJ (1942) The problem of bacteriophages in cheese making. Part I. Observations and investigations on slow acid production. J Dairy Res 13:58–72
Elliker PR, Frazier WC (1938) Factors affecting the activity and heat resistance of Swiss cheese starter cultures. I. Influence of time and temperature of incubation. J Dairy Sci 21:801–813
Hickey FC (1940) The ionization of lactic acid in aqueous sodium chloride solutions from 0 to 37.5°C. J Am Chem Soc 62:2916–2919
Horrall BE, Elliker PR (1950) An activity test for cheddar and cottage cheese starters. J Dairy Sci 33:245–249
Junker M, Liepe HU (1979) Determining the activity of starter cultures (pure cultures of lactobacilli). Fleischwirtschaft 59:1880–1881
Junker M, Liepe HU (1981) Influence magnitudes in determining the activity of lactobacilli starter cultures. Fleischwirtschaft 61:1980–1981
Man JC de, Rogosa M, Sharpe ME (1960) A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135
Murphey MG, Condon S (1984) Comparison of aerobic and anaerobic growth of Lactobacillus plantarum in a glucose medium. Arch Microbiol 138:49–53
Pearce LE (1969) Activity test for cheese starter cultures. NZJ Dairy Technol 4:246–247
Schleifer KH (1987) Recent changes in taxonomy of lactic acid bacteria. FEMS Microbiol Rev 46:201–203
Stadhouders J (1980a) Description of a method for determining the activity of cheese starters. Int Dairy Fed Annu Bull 129:9–10
Stadhouders J (1980b) Factors affecting the results of an activity test of mesophilic cheese starters. Int Dairy Fed Annu Bull 129:5–8
Weast RC, Astle MJ (eds) (1979) Handbook of chemistry and physics. 59th edn. CRC Press, Boca Raton, pp D203-D204
Whitehead HR, Cox GA (1932) A method for determination of vitality in starters. NZJ Sci Technol 13:304–309
Willis CJ (1981) Another approach to titration curves. J Chem Educ 58:659–663
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Lievense, L.C., van't Riet, K. & Noomen, A. Measuring and modelling the glucose-fermenting activity of Lactobacillus plantarum . Appl Microbiol Biotechnol 32, 669–673 (1990). https://doi.org/10.1007/BF00164737
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DOI: https://doi.org/10.1007/BF00164737