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Oxygen and temperature effects on acetaldehyde-induced catabolite inactivation in Pichia pastoris

  • Applied Microbial and Cell Physiology
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Summary

Exposure of methylotrophic yeasts to other carbon sources after growth on methanol results in catabolite inactivation. As a result, peroxisomes are rapidly degraded effectively disabling the metabolic pathway initiated by alcohol oxidase in favour of a more energetically favourable route. A model equation has been developed to describe the effect of temperature, dissolved oxygen concentration and acetaldehyde (catabolite) concentration on catabolite inactivation in Pichia pastoris. When pre-exposed to 4 g/l acetaldehyde at 30°C, the rate of conversion of ethanol to acetaldehyde decreased by 75%. Inactivation was reduced to 45% at 30°C by reducing the dissolved oxygen concentration. At high dissolved oxygen concentration, enzyme function was only inactivated by 20% if the temperature during the period of exposure to acetaldehyde was reduced to 5°C. The influence of acetaldehyde can be eliminated completely by operating at 5°C and low dissolved oxygen concentrations. Application of these findings to process design has enabled us to conduct preliminary reactions in laboratory-scale reactors that have yielded acetaldehyde concentrations greater than 3 M (130 g/l) in 4 h.

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Authors and Affiliations

  1. Energy Mines and Resources Canada, Alternative Energy Division-CANMET, 580 Booth Street, Room 774, K1A 0E4, Ottawa, Ontario, Canada

    Sheldon J. B. Duff

  2. Health and Welfare Canada, Pesticides Division, Environmental Health Centre, K1A 0L2, Ottawa, Ontario, Canada

    William D. Murray

  3. National Research Council of Canada, Division of Biological Sciences, 100 Sussex Drive, K1A 0R6, Ottawa, Canada

    Ralph P. Overend

Authors
  1. Sheldon J. B. Duff
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  2. William D. Murray
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  3. Ralph P. Overend
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Duff, S.J.B., Murray, W.D. & Overend, R.P. Oxygen and temperature effects on acetaldehyde-induced catabolite inactivation in Pichia pastoris . Appl Microbiol Biotechnol 36, 82–86 (1991). https://doi.org/10.1007/BF00164703

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  • DOI: https://doi.org/10.1007/BF00164703

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