Skip to main content
SpringerLink
Log in

The lack of an effect of intraocular steroids on irradiated fibroblasts in experimental proliferative vitreoretinopathy

  • Laboratory Investigations
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

Contraction of intraocular membranes is an important event in the development of proliferative vitreoretinopathy (PVR). When sufficient numbers of cells are present in the vitreous cavity, the retina usually detaches as a result of the contractive force generated by these cells. Steroids reduce the occurrence of retinal detachments in rabbit models of PVR by inhibiting the proliferation of injected fibroblasts. In this study, we used non-proliferative, irradiated cells to determine a possible effect of steroids on preretinal membrane contraction in PVR. We found no clinical difference between steroid treated eyes and sham-treated control eyes. Surgical reduction of the contractile tissue and medical therapy to prevent reproliferation are necessary in order to treat PVR effectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Algvere P, Koch E (1976) Experimental fibroplasia in the rabbit vitreous. Retinal detachment induced by autologous fibroblasts. Graefe's Arch Clin Exp Ophthalmol 199:215–222

    Google Scholar 

  2. Chandler DB, Rozakis G, deJuan E Jr, Machemer R (1985) The effect of triamcinolone acetonide on a refined model of proliferative vitreoretinopathy. Am J Ophthalmol 99:686–690

    Google Scholar 

  3. Chandler DB, Quansah FA, Hida T, Machemer R (1986) A refined experimental model for proliferative vitreoretinopathy. Graefe's Arch Clin Exp Ophthalmo1 224:86–91

    Google Scholar 

  4. Chandler DB, Hida T, Sheta SM, Proia AD, Machemer R (1987) Improvement in efficacy of corticosteroid therapy in an animal model of proliferative vitreoretinopathy by pretreatment. Graefe's Arch Clin Exp Ophthalmo1 225:259–265

    Google Scholar 

  5. Dannenberg AM Jr (1979) The anti-inflammatory effects of glucocorticosteroids: a brief review of the literature. Inflammation 3:329–343

    Google Scholar 

  6. Fastenberg DM, Diddie KR, Dorey K, Ryan SJ (1982) The role of cellular proliferation in an experimental model of massive periretinal proliferation. Am J Ophthalmol 93:565–572

    Google Scholar 

  7. Hatchell DL, McAdoo T, Sheta SM, King RT, Bartolome JV (1988) Quantification of cellular proliferation in experimental proliferative vitreoretinopathy. Arch Ophthalmol 106:669–672

    Google Scholar 

  8. Hida T, Chandler DB, Sheta SM (1987) Classification of the stages of proliferation vitreoretinopathy in a refined experimental model in the rabbit eye. Graefe's Arch Clin Exp Ophthalmol 225:303–307

    Google Scholar 

  9. Hitchens CA, Grierson I, Hiscott PS (1985) The effects of injections of cultured fibroblasts in the rabbit vitreous. Graefe's Arch Clin Exp Ophthalmol 223:237–249

    Google Scholar 

  10. Ponec M (1984) Effects of glucocorticoids on cultured human skin fibroblasts and keratinocytes. Int J Dermatol 23:11–24

    Google Scholar 

  11. Puck TT, Marcus PI (1955) A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: The use of x-irradiated cells to supply conditioning factors. Proc Nall Acad Sci USA 41:432–437

    Google Scholar 

  12. Puck TT, Marcus PIO, Cieciura SJ (1956) Clonal growth of mammalian cells invitro: growth characteristics of colonies from single HeLa cells with and without a “feeder” layer. J Exp Med 103:273–284

    Google Scholar 

  13. Ruhmann AG, Berliner DL (1967) Influence of steroids on fibroblasts. II. The fibroblast as an assay system for topical anti-inflammatory potency of corticosteroids. J Invest Dermatol 49:123–130

    Google Scholar 

  14. Sugita G, Tano Y, Machemer R, Abrams G, Claflin A, Fiorentino G (1980) Intravitreal autotransplantation of fibroblasts. Am J Ophthalmol 89:121–130

    Google Scholar 

  15. Tano Y, Chandler DB, Machemer R (1980) Treatment of intraocular proliferation with intravitreal injection of triamcinolone acetonide. Am J Ophthalmol 90:810–816

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Ophthalmology, Duke University Eye Center, Box 3802, 27710, Durham, NC, USA

    David B. Chandler, Tetsuo Hida, George Rozakis, Valorie S. Forbes & Robert Machemer

Authors
  1. David B. Chandler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tetsuo Hida
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. George Rozakis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Valorie S. Forbes
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Robert Machemer
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Offprint requests to: R. Machemer

This study was supported by the National Eye Institute, Grant EYO 2903, Research to Prevent Blindness, Alcon Laboratories, and the Helena Rubinstein Foundation

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chandler, D.B., Hida, T., Rozakis, G. et al. The lack of an effect of intraocular steroids on irradiated fibroblasts in experimental proliferative vitreoretinopathy. Graefe's Arch Clin Exp Ophthalmol 230, 188–191 (1992). https://doi.org/10.1007/BF00164662

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00164662

Keywords

Search

Navigation