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Expression of tenascin and fibronectin in the rabbit cornea after excimer laser surgery

Graefe's Archive for Clinical and Experimental Ophthalmology volume 230, pages 178–183 (1992)Cite this article

Abstract

In order to investigate the effects of excimer laser surgery on corneal wound healing, 25 rabbits underwent anterior keratectomy at a depth of 100 or 300 μm with a Meditec MEL 50 excimer laser. After various intervals the animals were killed and the cornea excised and investigated immunohistochemically for the expression of extracellular matrix (ECM) proteins, fibronectin and tenascin. Fibronectin was shown to occur earlier than tenascin, and the two also had different distribution patterns. Wound depth showed no clear effect on the localization and time of ECM protein expression. This study indicates that corneal wounds caused by excimer laser radiation and those caused by mechanical surgery differ as to healing mechanisms.

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Affiliations

  1. Department of Ophthalmology, University of Helsinki, Haartmaninkatu 4 C, SF-00290, Helsinki, Finland

    Gysbert -B. van Setten, Jürgen W. Koch, Timo Tervo & Ahti Tarkkanen

  2. Department of Anatomy, University of Helsinki, Haartmaninkatu 4 C, SF-00290, Helsinki, Finland

    Kaarina Tervo & Ismo Virtanen

  3. Department of Ophthalmology and Eye Hospital, University of Erlangen-Niirnberg, Schwabachanlage 6, W-8520, Erlangen, Federal Republic of Germany

    Gerhard K. Lang, Gottfried O. H. Naumann & Jörn Kolkmeier

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  1. Gysbert -B. van Setten
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  2. Jürgen W. Koch
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  3. Kaarina Tervo
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  4. Gerhard K. Lang
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  5. Timo Tervo
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  6. Gottfried O. H. Naumann
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  7. Jörn Kolkmeier
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  8. Ismo Virtanen
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  9. Ahti Tarkkanen
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Offprint requests to: G.-B. van Setten

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van Setten, G.B., Koch, J.W., Tervo, K. et al. Expression of tenascin and fibronectin in the rabbit cornea after excimer laser surgery. Graefe's Arch Clin Exp Ophthalmol 230, 178–183 (1992). https://doi.org/10.1007/BF00164660

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  • DOI: https://doi.org/10.1007/BF00164660

Keywords

  • Public Health
  • Radiation
  • Protein Expression
  • Extracellular Matrix
  • Wound Healing