Abstract
Nerve fiber layer height measured with respect to a standardized retinal reference plane is diminished by glaucoma. The definition of the reference plane influences the nerve fiber layer measurements. We empirically determined the best reference plane for measurement of nerve fiber layer height. Optimal parameters for measurement reproducibility were determined for a group of 6 normal and 6 glaucomatous eyes each imaged nine times. Optimal ability to distinguish normal from glaucomatous eyes was determined for a group of 33 normal eyes and 36 glaucomatous eyes each imaged once. Measurements with the smallest variability (root mean square error = 32 gm) and the highest sensitivity (83%) and specificity (88%) were achieved when the reference plane is defined by portions of the image from a peripheral temporal area 32° wide, and for two peripheral nasal areas of 55° width centered 30° above and below horizontal. These parameters for the definition of the reference plane should provide measurements of nerve fiber layer height with the least variability and the greatest ability to discriminate between eyes with early glaucomatous damage and normal eyes.
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References
Airaksinen PJ, Drance SM, Douglas GR, Mawson DK (1984) Diffuse and localized nerve fiber loss in glaucoma. Am J Ophthalmol 98:566–571
Algazi VR, Keltner JL, Johnson CA (1985) Computer analysis of the optic cup in glaucoma. Invest Ophthalmol Vis Sci 12:1759–1770
Armaly MF (1969) The correlation between appearance of the optic cup and visual function. Trans Am Acad Ophthalmol Otolaryngol 73:898–913
Bishop KI, Werner EB, Krupin T, Kozart DM, Beck SR, Nunan FA, Wax MB (1988) Variability and reproducibility of optic disc topographic measurements with the Rodenstock Optic Nerve Head Analyzer. Am J Ophthalmol 106:696–702
Caprioli J (1990) The contour of the juxtapapillary nerve fiber layer in glaucoma. Ophthalmology 97:358–366
Caprioli J, Miller JM (1988) Videographic measurement of optic nerve topography in glaucoma. Invest Ophthalmol Vis Sci 29:1294–1298
Caprioli J, Miller JM (1989) Measurement of relative nerve fiber layer surface height in glaucoma. Ophthalmology 96:633–639
Caprioli J, Ortiz-Colberg R, Miller JM, Tressler C (1989) Measurements of peripapillary nerve fiber layer contour in glaucoma. Am J Ophthalmol 108:404–413
Cornsweet TN, Hersh S, Humphries JC, Beesmer RJ, Cornsweet DW (1983) Quantification of the shape and color of the optic nerve head. In: Breinin GM, Siegel IM (eds) Advances in diagnostic visual optics. Springer, New York Berlin Heidelberg, pp 141–149
Dandona L, Quigley HA, Jampel HD (1989) Reliability of optic nerve head topographic measurements with computerized image analysis. Am J Ophthalmol 108:414–421
Eikelboom RH, Cooper RL, Barry CJ (1990) A study of variance in densitometry of retinal nerve fiber layer photographs in normals and glaucoma suspects.Invest Ophthalmol Vis Sci 31:2373–2383
Hamming RW (1979) Numerical methods for scientists and engineers. McGraw-Hill, New York, pp 349–355
Hoyt WF, Frisen L, Newman NM (1973) Fundoscopy of nerve fiber layer defects in glaucoma. Invest Ophthalmol 12:814–829
Lichter PR (1976) Variability of expert observers in evaluating the optic disc. Trans Am Ophthalmol Soc 64:532–572
Mikelberg FS, Douglas GR, Schulzer M, Airaksinen PJ, Wijsman K, Mawson D (1986) The correlation between cup-disk ratio, neuroretinal rim area, and optic disk area measured by the video-ophthalmograph (Rodenstock Analyzer) and clinical measurement. Am J Ophthalmol 101:7–12
Read RM, Spaeth GL (1975) The practical clinical appraisal of the optic disc in glaucoma: the natural history of cup progression and some specific disc-field correlations. Trans Am Acad Ophthalmol Otolaryngol 78:OP255-OP274
Sommer A, Katz J, Quigley HA, Miller NR, Robin AL, Richter RC, Witt K (1991) Clinically detectable nerve fiber layer atrophy precedes the onset of glaucomatous field loss. Arch Ophthalmol 109:77–83
Takamoto T, Schwartz B (1989) Photogrammetric measurement of nerve fiber layer thickness. Ophthalmology 96:1315–1319
Varma R, Spaeth GL, Steinmann WC, Katz LJ (1989) Agreement between clinicians and an image analyzer in estimating cup-to-disc ratios. Arch Ophthalmol 107:526–529
Weinreb RN, Dreher AW, Bille JF (1989) Quantitative assessment of the optic nerve head with the laser tomographic scanner. Int Ophthalmol 13:25–29
Weinreb RN, Dreher AW, Coleman A, Quigley H, Shaw B, Reiter K (1990) Histopathologic validation of Fourier-ellipsometry measurements of retinal nerve fiber layer thickness. Arch Ophthalmol 108:557–560
Wirtschafter JD (1983) Optic nerve axons and acquired alterations in the appearance of the optic disc. Trans Am Ophthalmol Soc 81:1034–1091
Zeimer RC, Mori MT, Bahrain K (1989) Feasibility test of a new method to measure retinal thickness noninvaseively. Invest Ophthalmol Vis Sci 30:2099–2105
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Miller, J.M., Caprioli, J. An optimal reference plane to detect glaucomatous nerve fiber layer abnormalities with computerized image analysis. Graefe's Arch Clin Exp Ophthalmol 230, 124–128 (1992). https://doi.org/10.1007/BF00164649
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DOI: https://doi.org/10.1007/BF00164649