Applied Microbiology and Biotechnology

, Volume 44, Issue 1–2, pp 172–176 | Cite as

Storage of lyophilized cultures of Lactobacillus bulgaricus under different relative humidities and atmospheres

  • H. P. Castro
  • P. M. Teixeira
  • R. Kirby
Applied Microbial and Cell Physiology


The viability of lyophilized cultures of Lactobacillus bulgaricus in skim milk, during storage at different temperatures, relative humidities, and atmospheres was investigated. Survival was greatest at 11% relative humidity and at 5°C. Indirect and direct evidence is presented supporting the hypothesis that membrane damage occurs during storage. Experiments on the lipid composition of the cell membrane demonstrate that changes occur with time that are probably the result of oxidation. A study on the lipid composition of the cell membrane by gas chromatography showed that the unsaturated/saturated fatty acid index changes with time during storage.


Oxidation Lipid Atmosphere Chromatography Cell Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • H. P. Castro
    • 1
  • P. M. Teixeira
    • 1
  • R. Kirby
    • 1
  1. 1.Escola Superior de BiotecnologiaPortoPortugal

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