Summary
A denitrifying bacterium showing typical characteristics of Pseudomonas sp. (Al1) capable of growth on 1-heptadecene as the sole source of carbon and energy has been isolated from a hydrocarbon-polluted marine sediment by using classical enrichment techniques. The generation time for anaerobic growth on 1-heptadecene was 24 h, and the percentage of hydrocarbon degradation under anaerobic conditions ranged from 19 to 23%. The emulsifying capacity was observed and suggested that Al1 cultivated anaerobically on heptadecene produced surface-active agents.
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References
Atlas RM (ed) (1984) Petroleum microbiology. Macmillan, New York
Azoulay E, Chouteau J, Davidovics G (1963) Isolement et caractérisation des enzymes responsables de l'oxydation des hydrocarbures. Biochim Biophys Acta 77:554–567
Bauman P, Bauman L (1981) The Gram-negative eubacteria: genus Photobacterium, Benekea, Alteromonas, Pseudomonas and Alcaligenes. In: Mortimer PS, Heinz S, Trupper HG, Ballows A, Shleger HG (eds) The prokaryotes. A handbook on habitats, isolation and identification of bacteria. Springer, Berlin Heidelberg New York, pp 1304–1330
Bendschneider K, Robinson RJ (1972) A new spectrometric method for determination of nitrite in sea-water. J Mar Res 11:87–96
Bertrand JC, Caumette P, Mille G, Gilewicz M, Denis M (1989) Anaerobic biodegradation of hydrocarbons. Sci Prog Oxf 73:333–350
Bonin P, Gilewicz M, Bertrand JC (1989) Effects of oxygen on each step of denitrification on Pseudomonas nautica. Can J Microbiol 35:1061–1064
Chan YK, Knowles R (1979) Measurement of denitrification in fresh water sediments by an in-situ acetylene inhibition method. Appl Environ Microbiol 37:1076–1072
Chouteau J, Azoulay E, Senez JC (1962) Anaerobic formation of n-hept-1-ene from n-heptane by resting cells of Pseudomonas aeruginosa. Nature 194:576–578
Davis JB, Yarbrough HP (1966) Anaerobic oxidation of hydrocarbons by Desulfovibrio desulfuricans. Chem Geol 1:137–144
Dolfing J, Zeyer J, Binder-Eicher P, Schwarzenbach RR (1990) Isolation and characterization of a bacterium that mineralizes toluene in the absence of molecular oxygen. Arch Microbiol 154:336–341
Hansen RW, Kallio RE (1967) Inability of nitrate to serve as a terminal oxidant for hydrocarbons. Science 125:1198–1199
Hochstein LI, Betlach M, Kritikos G (1984) The effect of oxygen on denitrification during steady state growth of Paracoccus halodenitrificans. Arch Microbiol 137:74–78
Iida M, Iizuka H (1970) Formation of n-decene-1 from n-decane by resting cells of Candida rugosa. Z Allg Mikrobiol 9:223–226
Iizuka H, Iida M, Fujita S (1969) Anaerobic formation of n-decyl alcohol from n-decene-1 by resting cells of Candida rugosa. Z Allg Mikrobiol 10:245–252
Klug MJ, Markovetz AJ (1971) Utilization of aliphatic hydrocarbons by micro-organisms. Avd Microbiol Physiol 5:1–43
Larsen H (1986) Halophilic and halotolerant microorganisms: a review and historical perspective. FEMS Microbiol Res 39:3–7
Lloyd D, Boddy L, Davies KJ (1987) Persistence of bacterial denitrification under aerobic conditions: the rule rather than the exception. FEMS Microbiol Ecol 45:185–190
MacAulife L (1971) GC determination of solutes by multiple phase equilibrium. Chem Technol 1:46–51
Mattei G, Rambeloarisoa E, Giusti G, Rontani JF, Bertrand JC (1986) Fermentation procedure of a crude oil in continuous culture on sea water. Appl Microbiol Biotechnol 23:302–304
Mille G, Chen JY, Dou H, Azoulay E, Colin M, Bertrand JC (1985) Evolution des hydrocarbures dans les sédiments côtiers méditerranéens prélevés à proximité d'un émissaire d'une raffinerie de pétrole. Mar Environ Res 17:65–80
Oberbremer A, Muller-Hurtig R, Wagner F (1990) Effect of the addition of microbial surfactants on hydrocarbon degradation in a soil population in a stirred reactor. Appl Microbiol Biotechnol 32:485–489
Robertson LA, Kuenen JG (1984a) Aerobic denitrification, old wine in new bottles. Antonie van Leeuwenhoek 50:525–544
Robertson LA, Kuenen JG (1984b) Aerobic denitrification, a controversy revived. Arch Microbiol 139:351–354
Rontani JF, Giusti G (1986) Study of the biodegradation of polybranched alkanes by a marine bacterial community. Mar Chem 20:197–205
Rosenberg E (1986) Microbial surfactants. CRC Crit Rev Biotechnol 3:109–132
Roy PK, Singh HD, Bhagat SD, Barnah JN (1979) Characterization of hydrocarbon emulsification and solubilization occuring during the growth of Endomycopsis lipolytica on hydrocarbon. Biotechnol Bioeng 21:955–9
Saliot A (1981) Natural hydrocarbons in sea water. In: Duursma EK, Dawson R (eds) Marine organic chemistry. Elsevier Scientific Publishing Company, Amsterdam, pp 327–374
Shink B (1985a) Fermentation of acetylene by an obligate anaerobic Pelobacter acetylenicus sp nov. Arch Microbiol 142:295–301
Shink B (1985b) Degradation of unsaturated hydrocarbons by methanogenic enrichment. FEMS Microbiol Ecol 31:69–77
Treguer P, Le Corre P (1975) Manuel d'analyse des sels nutritifs dans l'eau de mer Laboratoire d'Océanologie chimique. Université de Bretagne Occidentale, Brest, France, pp 120
Wagner F, Zahn W, Buhring U (1967) 1-Hexadecene, an intermediate in the microbial oxidation of n-hexadecane in-vivo and in-vitro. Angew Chem Internat Edit 6:359–360
Wambeke F van (1988) Numération et taille des bactéries planctoniques au moyen de l'analyse d'images couplée à l'épiflourescence. Ann Inst Pasteur Microbiol 130:261–272
Widdel F (1988) In: Zehnder AJB (ed) Biology of anaerobic microorganisms. Wiley, New York, pp 469–585
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Gilewicz, M., Monpert, G., Acquaviva, M. et al. Anaerobic oxidation of 1-n-heptadecene by a marine denitrifying bacterium. Appl Microbiol Biotechnol 36, 252–256 (1991). https://doi.org/10.1007/BF00164430
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DOI: https://doi.org/10.1007/BF00164430