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Oligoclonal expansion of Vβ8+ cells in interleukin-2-activated T cells residing in subcutaneous metastatic melanoma

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Abstract

Oligoclonality was investigated in interleukin-2 (IL-2)-activated T cells (tumor-inrdtrating lymphocytes, TILs) residing in metastatic melanomas using seven different monoclonal antibodies (mAbs) specific for T-cell receptor (TCR) Vα or Vβ regions and flow cytometry. IL-2-activated TILs from 25 of 42 metastatic melanomas (60%) displayed oligoclonal expansion, whereas IL-2-activated peripheral bIIod mononuclear cells (PBMCs) from only 2 of 20 patients (10%) did so during 2–5 weeks in culture. Skin-derived lymphocytes from 20 patients were cultured; only four samples proliferated and none showed oligoclonal expansion. Preferential oligoclonal expansion of TILs was observed in Vβ8+ cells (10/42, P < 0.05), Vβ6.7+ cells (7/42, P < 0.05), and Vα2+ cells (7/42, not significant). Oligoclonal expansion of Vβ8+ cells was primarily found in T cells from subcutaneous metastases (8/20 cases, P < 0.05), whereas that of Vβ6.7+ cells and Vα2+ cells was also found in T cells from lymph node or organ metastases. These mAbs to TCR V regions stimulated effector TILs to produce interferon-γ, but not IL-2 or IL-4. Subcutaneous tumor-specific (Vβ8+ cells) and non-specific (Vβ6.7+ cells and Vα2+ cells) oligoclonalities were observed in IL-2-activated melanoma TILs, suggesting different immune responses among different sites of metastases.

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Authors and Affiliations

  1. Departments of Immunology, The University of Texas MD, Anderson Cancer Center, 77030, Houston, TX, USA

    T. Morita, M. A. Salmeron & K. Itoh

  2. Department of Neurosurgery, The University of Texas MD, Anderson Cancer Center, 77030, Houston, TX, USA

    R. P. Moser

  3. General Surgery, The University of Texas MD, Anderson Cancer Center, 77030, Houston, TX, USA

    M. I. Ross

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  1. T. Morita
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  2. M. A. Salmeron
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  3. R. P. Moser
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  4. M. I. Ross
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  5. K. Itoh
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Morita, T., Salmeron, M.A., Moser, R.P. et al. Oligoclonal expansion of Vβ8+ cells in interleukin-2-activated T cells residing in subcutaneous metastatic melanoma. Clin Exp Metast 10, 69–76 (1992). https://doi.org/10.1007/BF00163578

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  • DOI: https://doi.org/10.1007/BF00163578

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