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Improved cultivation of polarized animal cells on culture inserts with new transparent polyethylene terephthalate or polycarbonate microporous membranes

  • Animal Cell Culture
  • Published:
Biotechnology Techniques

Summary

CaCo 2 and MDCK epithelial cells, cultivated on transparent polyethylene terephthalate or polycarbonate Cyclopore microporous membranes, adhere as good, grow and differentiate faster than on tissue culture polystyrene or Millicell CM culture inserts. The rate of growth of MDCK cells is slightly increased by raising pore density or diameter of PETP membranes, whereas that of CaCo 2 cells is decreased when pore diameter is raised.

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Authors and Affiliations

  1. Laboratoire de Biochimie, Pl. Pasteur, 1, Université Catholique de Louvain, 1348, Louvain-La-Neuve, Belgium

    Thérèse Sergent-Engelen, Christine Halleux & Yves-Jacques Schneider

  2. Laboratoire des Hauts Polymères Pl. Croix du Sud, 1, Université Catholique de Louvain, 1348, Louvain-La-Neuve, Belgium

    Etienne Ferain, Henri Hanot & Roger Legras

Authors
  1. Thérèse Sergent-Engelen
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  2. Christine Halleux
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  3. Etienne Ferain
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  4. Henri Hanot
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  5. Roger Legras
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  6. Yves-Jacques Schneider
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Sergent-Engelen, T., Halleux, C., Ferain, E. et al. Improved cultivation of polarized animal cells on culture inserts with new transparent polyethylene terephthalate or polycarbonate microporous membranes. Biotechnol Tech 4, 89–94 (1990). https://doi.org/10.1007/BF00163279

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  • DOI: https://doi.org/10.1007/BF00163279

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