Improved cultivation of polarized animal cells on culture inserts with new transparent polyethylene terephthalate or polycarbonate microporous membranes

Summary

CaCo 2 and MDCK epithelial cells, cultivated on transparent polyethylene terephthalate or polycarbonate Cyclopore microporous membranes, adhere as good, grow and differentiate faster than on tissue culture polystyrene or Millicell CM culture inserts. The rate of growth of MDCK cells is slightly increased by raising pore density or diameter of PETP membranes, whereas that of CaCo 2 cells is decreased when pore diameter is raised.

This is a preview of subscription content, access via your institution.

References

  1. Caplan, M.J., Anderson, H.C., Palade, G.E., and Jamieson, J.D. (1986). Cell, 46, 623–631.

    Google Scholar 

  2. Chantret, I., Barbat, A., Dussaulx, E., Brattain, M.G., and Zweibaum, A. (1988). Cancer Res., 48, 1936–1942.

    Google Scholar 

  3. Halleux, C., and Schneider, Y.-J. (1988). Cytotechnology, S 67.

  4. Heth, C.A., Yankauckas, M.A., Adamian, M., and Edwards, R.B. (1987). Cur. Eye Res., 6, 1007–1019.

    Google Scholar 

  5. Hidalgo, I.J., Raub, T.J., and Borchardt, R.T. (1989). Gastroenterol., 96, 736–749.

    Google Scholar 

  6. Kristensen, T., Mortensen, T., and Nissen, N. (1985). Exp. Hematol, 13, 1188–1191.

    Google Scholar 

  7. Legras, R., and Jongen, Y. Fr. Patent n∘W 086/04263.

  8. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951). J. Biol. Chem. 265–275.

  9. Pitt, A.M., Gabriels, J.E., Badmington, F., McDowell, J., Gonzales, L., and Waugh, M.E. (1987). Biotechniques, 5, 162–171.

    Google Scholar 

  10. Ramond, M., Martinot-Peignoux, M., and Erlinger, S. (1985). Biol. Cell, 54, 89–92.

    Google Scholar 

  11. Schneider, Y.-J. (1989). J. Immunol. Meth., 116, 65–77.

    Google Scholar 

  12. von Bonsdorff, C., Fuller, S.D., and Simons, K. (1985). EMBO J., 4, 2781–2792.

    Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sergent-Engelen, T., Halleux, C., Ferain, E. et al. Improved cultivation of polarized animal cells on culture inserts with new transparent polyethylene terephthalate or polycarbonate microporous membranes. Biotechnol Tech 4, 89–94 (1990). https://doi.org/10.1007/BF00163279

Download citation

Keywords

  • Epithelial Cell
  • Polyethylene
  • Tissue Culture
  • Polystyrene
  • CaCo