Abstract
In 106 clinical isolates of multiresistant Klebsiella pneumoniae strains, we found that aminoglycoside-resistance was due mostly to two adenylating enzymes: AAD (2″) (56.6%), that modifies gentamicin, kanamycin, tobramycin and sissomicin, and AAD (3″) 9 (56.6% + 19.8%) that modifies streptomycin and spectinomycin. The identification of these enzymes was possible by MICs determination against a set of aminoalycosides antibiotics. AAD (2″) + AAD (3″) 9 were coded by conjugative plasmid of about 120 Md.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
AajC. and BorstP. (1972): The gel electrophoresis of DNA. - Biochem. Biophys. Acta. 269, 192–198.
BenvenisteR. and DaviesJ. (1973): Mechanisms of antibiotic resistance in bacteria. - Annu. Rev. Biochem. 42, 471–506.
CasewellM.W., TalsaniaH.G. and KnightS. (1981) Gentamicin-resistant Klebsiella aerogenes as a clinically-significant source of transferable antibiotic resistance. - J. Antimicrob. Chemother. 8, 153–160.
CoetzeeJ.N., DattaN. and HedgesR.W. (1977): R-factors from Proteus rettgeri. - J. Gen. Microbiol. 72, 543–552.
CookeE.M., BraysonJ.B., EdmondsonA.S. and HallD. (1979): An investigation into the incidence into the incidence and sources of Klebsiella infections in hospital patients. - J. Hyg. Camb. 82, 473–480.
CurieK., SpellerD.C.E., SimpsonR.A., StephensM. and CookeD.I. (1978): A hospital epidemic caused by a gentamicin-resistant Klebsiella aerogenes. -J. Hyg. Camb. 80, 115–123.
DaviesD. and SmithD.J. (1978): Plasmid-determined resistance to antimicrobial agents. - Annu. Rev. Microbiol. 32, 469–518.
DrasarF.A. (1978): Detection of aminoglycoside degrading enzymes. In ≪Laboratory methods in antimicrobial chemotherapy≫ (Reeves D.S., Phillips I., Williams J.D. and Wise R.) (Eds) pp. 70–75. Churchill Livingstone, Edinburgh.
EckardtT. (1978): A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. - Plasmid 1, 582–584.
Facinelli B., Montanari M.P., Palmieri M. and Calegari L. (1983): ≪ Presenza di plasmidi coniugativi in ceppi multiresistenti di Klebsiella pneumoniae. -(In Press. Igiene Moderna).
FosterT.J. (1983): Plasmid-determined resistance to antimicrobial drugs and toxic metal ions in bacteria. - Microbiol. Rev. 47, 361–409.
Gavan T.L. and Barry A.L. (1980): Microdilution test procedures, pp. 459 in Manual of Clinical Microbiology, E.H. Lennette, ASM 1913 I St, N.W. Washington, D.C. 20006.
GerdingD.N., BuxtonA.E., HughesR.A., ClearyP.P., ArbaczawskiJ. and StammW.E. (1979): Nosocomial multiply resistant Klebsiella pneumoniae: epidemiology of an outbreak of apparent index case origin. - Antimicrob. Agents Chemother. 15, 608–615.
GrinterN.J. and BarthP.T. (1976): Characterization of SmSu plasmids restriction endonuclease cleavage and compatibility testing. - J. Bacteriol. 128, 394–400.
HassM.J. and DowdingJ.E. (1975): Aminoglycoside-modifying enzymes. - Methods Enzymol. 43, 611–628.
KadoC.I. and LiuS.T. (1981): Rapid procedure for detection and isolation of large and small plasmids. - J. Bacteriol. 145, 1365–1373.
KawabeH., TanakaT. and MitsuhashiS. (1978): Streptomycin and spectinomycin resistance mediated by plasmids. - Antimicrobial. Agents Chemother. 13, 1031–1035.
MaM.Y., GoldsteinE.J.C., FriedmanM.H., AndersonM.S. and MulliganM.E. (1983): Resistance of Gram-negative bacilli as related to hospital use of antimicrobial agents. - Antimicrob. Agents Chemother. 24, 347–352.
Alaniatis T., Fritsch tE.F. and Sambrook J. (1982): Large-scale isolation of plasmid DNA pp. 86, in ≪Molecular Cloning≫. - Eds. by CSH, New York 11724.
MillerG.H., SebatelliF.J., HareR.S. and WaitzJ.A. (1980): Survey of aminoglycoside resistance pattern. -Dev. Ind. Microb. 21, 91–104.
MillerJ.H. (1977): Curing of episomes, from Escherichia coli strains with Acridin Orange. pp. 104, in ≪Experiments in mot-ocular genetics≫. Eds. by CSH, New York 11724.
NagateT., InqueM., InqueK. and MitsuhashiS. (1978): Plasmid-mediated sulfanilamide resistance.- Microbiol. Immunol. 22, 367–375.
RennieR.P. and DuncanI.B. (1977): Emergence of gentamicin-resistant Klebsiella in a general hospital.- Antimicrob. Agents Chemother. 11, 179–183.
ReynoldsA.V. and SmithJ.T. (1979): Enzymes which modify aminoglycoside antibiotics. pp. 165–181, in Recent advances in infection, Vol. I (Reeves D.S. and Gedds A., Eds.) Churchill Livingstone, Edinburg.
RichardsH., HughesV. and DattaN. (1981): Diversity of plasmids responsible for multiple resistance in Klebsiella serotype K2. - J. Hyg. Camb. 86, 189–194.
ShannonK.P., PhillipsI. and KingB.A. (1978): Aminoglycoside resistance among Enterobacteriaceae and Acinetobacter species. - J. Antimicrob. Chemother. 4, 131–142.
ShonnonK. and PhillipsI. (1982): Mechanisms of resistance to aminoglycoside in clinical isolates. -J. Antimicrob. Chemother. 9, 91–102.
SwedbergG. and SkoldO. (1980): Characterization of different plasmid-borne dihydropteroato synthases mediating bacterial resistance to sulfonamides.- J. Bacteriol. 142, 1–7.
ThiemkeW.A. and NathanD.M. (1978): Simultaneous nosocornial outbreaks caused by multiply resistant Klebsiella pneumoniae type 2 and 30. -J. Clip. Microbiol. 8, 769–771.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Facinelli, B., Montanari, M.P. & Calegari, L. Plasmid-specified aminoglycoside-modifying enzymes in clinical isolates of Klebsiella pneumoniae . Eur J Epidemiol 1, 48–53 (1985). https://doi.org/10.1007/BF00162312
Issue Date:
DOI: https://doi.org/10.1007/BF00162312