Abstract
An epidemiological survey of Rotavirus infections in a maternity unit was carried out between May 1983 and August 1984. Rotaviruses have been detected throughout the survey in 184 of 528 fecal samples obtained from 3 and 6 day-old newborns. Rotavirus excretion could be detected neither from the mothers nor from the medical attendants. Rotavirus RNA was analyzed by polyacrylamide gel electrophoresis. All the strains isolated exhibited the same electropherotype. This result contrasts with the genomic variability amongst Rotavirus strains usually observed in pediatric wards, and is in agreement with some other studies conducted in maternity wards; in these locations Rotavirus infection seems to have a special epidemic status as compared with other communities.
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BuitenwerfJ., Muilwijk-Van AlphenM., SchaapG.J.P. (1983): Characterization of Rotaviral RNA isolated from children with gastroenteritis in two hospitals in Rotterdam. - J. Med. Virol., 12, 71–78.
ChanockS.J., WenskeE.A., FieldsB.N. (1983): Human Rotaviruses and genome RNA. - J. Infect. Dis., 148, 49–50.
ChrystieJ.L., TotterdellB.M., BanatvalaJ.E. (1978): Asymptomatic endemic Rotavirus infections in the newborn. - Lancet, 1, 1176–1178.
DimitrovD.H., GrahamD.Y., LopezJ., MuchinikG., VelascoG., StenbagkW.A., EstesM.K. (1984): RNA electrophoretypes of human Rotaviruses from North and South America. - Bull. WHO, 62, 321.
ForsterP., PastorS. (1983): Epidemiology of human Rotaviruses as determined by electrophoresis of genome RNA. - Eur. J. Clin. Microbial., 2, 141–147.
HildrethC., ThomasM., RidgwayG.L. (1981): Rotavirus infection in an obstetric unit. - Brit. Med. J., 282, 231.
KeswickB.H., PickeringL.K., DupontH.L., WoodwardW.E. (1983): Survival and detection of Rotaviruses on environmental surfaces in day care centers. - Appl. Environ. Microbial., 46, 813–816.
LaemmliU.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. - Nature, 227, 680–685.
LourencoM.H., NicolasJ.C., CohenJ., ScherrerR., BricoutF. (1981): Study of human Rotavirus genome by electrophoresis: attempt to classification among strains isolated in France. - Ann. Virol. (Inst. Pasteur), 132E, 161–173.
MurphyA.M., AlbreyM.B., CreweE.B. (1977): Rotavirus infections of neonates. - Lancet, 2, 1149–1150.
NicolasJ.C., LourencoM.H., MarchalS., CohenJ., ScherrerR., BricoutF. (1983): Description of a very simplified method for Rotavirus dsRNA extraction.- Ann. Virol. (Inst. Pasteur), 134E, 135–139.
NicolasJ.C., PothierP., CohenJ., LourencoM.H., ThompsonR., GuimbaudP., ChenonA., DauvergneM., BricoutF. (1984): Survey of human rotavirus propagation as studied by electrophoresis of genomic RNA. - J. Infect. Dis., 149, 688–693.
SamadiA.R., HuqM.I., AhmedQ.S. (1982): Detection of Rotavirus in handwashings of attendants of children with diarrhoea. - Brit. Med. J., 286, 188.
ScherrerR., CorthierG., L'HaridonR. (1980): ELISA for the detection of Rotavirus and Rotavirus/antibody complexes in faeces. - Current Tropico in Vet, Med. and Animal Sci., 13, 40–48.
RodgerS.M., BishopR.F., BirchC., McLeanB., HolmesI.H. (1981): Molecular epidemiology of human Rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. - J. Clin. Microbial., 13, 272–278.
SchnaglR.D., RodgerS.M., HolmesI.H. (1981): Variation in human Rotavirus electropherotypes occurring between Rotavirus gastroenteritis epidemics in central Australia. - Infect. Immun., 33, 17–21.
TotterdellB.M., ChrystieI.L., BanatvalaJ.E. (1976): Rotavirus infections in a maternity unit. - Arch. Dis. Child., 51, 924–928.
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Garbag-Chenon, A., Brussieux, J., Boisivon, A. et al. Epidemiology of human rotaviruses in a maternity unit as studied by electrophoresis of genomic RNA. Eur J Epidemiol 1, 33–36 (1985). https://doi.org/10.1007/BF00162309
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DOI: https://doi.org/10.1007/BF00162309