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Tangential flow filtration — A new method for the separation of bacterial enzymes from cell debris

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Summary

The ability of tangential flow filtration to separate enzymes from cell debris in bacterial homogenates has been studied. Homogenates of two organisms which are difficult to clarify by centrifugation were tested. Ultrafiitration membranes gave very low enzyme yields in the filtrates. However, microfiltration membranes gave clear filtrates containing high yields of enzyme.

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Authors and Affiliations

  1. Microbial Technology Laboratory PHLS Centre for Applied Microbiology and Research, Porton Down, SP4 OJG, Salisbury, Wiltshire, UK

    Alan V Quirk & John R Woodrow

Authors
  1. Alan V Quirk
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  2. John R Woodrow
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Quirk, A.V., Woodrow, J.R. Tangential flow filtration — A new method for the separation of bacterial enzymes from cell debris. Biotechnol Lett 5, 277–282 (1983). https://doi.org/10.1007/BF00161129

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  • DOI: https://doi.org/10.1007/BF00161129

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