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Xylanase, CM-cellulase and Avicelase production by the thermophilic fungus Sporotrichum thermophile

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Summary

When wheat straw was used as carbon source, Sporotrichum thermophile produced large amounts of xylanase extracellularly in addition to CM-cellulase and Avicelase. These enzymes were isolated by alcohol precipitation, desalting and column chromatography. The molecular weights were estimated to be 25,000 65,000 and 84,000 for xylanase, CM-cellulase and Avicelase respectively. Serine and threonine were found to be the most abundant amino acids and these enzymes are very acidic proteins.

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Authors and Affiliations

  1. Chemical and Biochemical Engineering Faculty of Engineering Science, The University of Western Ontario, N6A 5B9, London, Ontario, Canada

    Argyrios Margaritis & Rosnina Merchant

  2. Division of Biological Sciences, National Research Council of Canada, K1A 0R6, Ottawa, Ontario, Canada

    Makoto Yaguchi

Authors
  1. Argyrios Margaritis
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  2. Rosnina Merchant
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  3. Makoto Yaguchi
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Margaritis, A., Merchant, R. & Yaguchi, M. Xylanase, CM-cellulase and Avicelase production by the thermophilic fungus Sporotrichum thermophile . Biotechnol Lett 5, 265–270 (1983). https://doi.org/10.1007/BF00161127

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  • DOI: https://doi.org/10.1007/BF00161127

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