Skip to main content
SpringerLink
Log in

Fermentation of a waste-product from the industrial processing of the lime (Citrus aurantifolia swingle) for vitamin B12 production

I.- Culture conditions for Propionibacterium shermanii and effect of 5,6-dimethylbenzimidazole as precursor

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

The purpose of this study was to investigate the possibility of utilizing the distillation residue, produced in the industrial processing of the lime, as a substrate for the production of vitamin B12 by Propionibacterium freudenreichii and Propionibacterium shermanii. Both microorganisms were found capable of fermenting the distillation residue with no significant differences in the production of this vitamin. However, P. shermanii was selected for the study since it presented fewer problems in the extraction and analysis of the vitamin.

After NaOH, Ca(OH)2, and NH4OH were used to adjust the pH of the medium for optimal growth of the propionibacteria strains, deficiencies in the media were corrected. NaOH was selected since it provided good yield without removing fermentation sources. Under anaerobic conditions, 5,6-dimethylbenzimidazole had a positive effect on the synthesis of vitamin B12; under anaerobic-aerobic conditions, a negative effect.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Berry, E.C. and Bullerman, L.B. (1966). Use of cheese whey for vitamin B12 production. II cobalt, precursor and aeration levels. Applied Microbiology 14, 3:353–355.

    Google Scholar 

  • Carpenter, P.L. (1969) Microbiología. Segunda Edición. Ed. Interamericana. España. pp. 361.

    Google Scholar 

  • Fideicomiso del Limón (1979) Folleto. Ed. Fideicomiso del Limón. México.

    Google Scholar 

  • Fisher, R.A. (1954) Rapid spectrophotometric determination of vitamin B12 in microbial material. J. Agr. Food. Chem. 1:951–953.

    Google Scholar 

  • Florent, J. and Ninet, L. (1979) vitamin B12. Microbial Technology, 2nd. Ed. Vol. 1 Chapter 15, 497–519. Academic Press. Inc. N.Y.

    Google Scholar 

  • Instituto Mexicano de Comercio Exterior (1981). México.

  • Lim, E.P.G. (1968) U.S. Patent 3,411,991.

  • Maitra, P.K. and Roy, S.C. (1960) Trace elements and the synthesis of vitamin B12 by Streptomyces olivaceus. Biochem. J. 75:483–487.

    Google Scholar 

  • Speedie, J.D. and Hull, G.W. (1960) U.S. Patent 2,951,017.

  • Wood, H.G., Andersen, A.A. and Werkman, C.H. (1938) Nutrition of the propionic acid bacteria. J. Bacteriol. 36:201–214.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Biotecnología del Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70228, 04510, México, D.F., México

    Fernando García-Hernández

  2. Escuela Nacional de Fruticultura de la CONAFRUT, SARH, México

    José Luis Pérez-Mendoza

Authors
  1. José Luis Pérez-Mendoza
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fernando García-Hernández
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pérez-Mendoza, J.L., García-Hernández, F. Fermentation of a waste-product from the industrial processing of the lime (Citrus aurantifolia swingle) for vitamin B12 production. Biotechnol Lett 5, 259–264 (1983). https://doi.org/10.1007/BF00161126

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00161126

Keywords

Search

Navigation