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Determination of the volume fraction of yeast cells in a sediment or in a suspension

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Summary

A simple and precise method for the measurement of the volume fraction of cells in a sediment or in a suspension is described. Determinations carried out with yeast cells sediments obtained from completely fermented molasses mash lead to cells volume fraction values affected by a coefficient of variation equal to 1.9% and to a cell density equal to 1.115 g.ml−1.When the method was applied to cells suspensions the coefficient of variation of the cells volume fraction was 2.9% and the cell density was 1.120 g.ml−1.

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Authors and Affiliations

  1. Division of Chemistry and Chemical Engineering, Instituto de Pesquisas Tecnológicas do Estado de São Paulo S.A.(IPT), C.P.7141, 01000, São Paulo, SP, Brazil

    W. Borzani

  2. Biochemical Engineering Laboratory, Department of Chemical Engineering, Escola Politécnica, University of São Paulo, C.P.8174, 01000, São Paulo, SP, Brazil

    Marina L. R. Vairo

Authors
  1. W. Borzani
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  2. Marina L. R. Vairo
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Borzani, W., Vairo, M.L.R. Determination of the volume fraction of yeast cells in a sediment or in a suspension. Biotechnol Lett 5, 247–252 (1983). https://doi.org/10.1007/BF00161124

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  • DOI: https://doi.org/10.1007/BF00161124

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