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Kinetic study on starch hydrolysis using a glucose/maltose sensing system

  • Published:
Biotechnology Techniques

Summary

A dual-enzyme electrode flow injection system that can simultaneously determine glucose and maltose is used for an on-line study of starch hydrolyses catalysed by amylases. With the working system, determinations can be made every 2 minutes. A 10 μL sample size with recycled back-flow minimises any loss of the reaction medium. The production, growth and decay of glucose and maltose concentrations during starch hydrolysis under various enzymatic conditions can thus be closely monitored, making it useful for the study of the catalytic kinetics of amylases and in screening and analysing enzyme systems.

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References

  • Bertrand, C., Coulet, P.R. and Gautheron, D. C.(1981), Anal. Chim. Acta. 126, 23–24.

    Google Scholar 

  • Converti, A., Fiorito, G., Del Borghi, M. and Ferraiolo, G. (1991), Bioprocess Eng. 7, 165–170.

    Google Scholar 

  • Cordonnier, M., Lawng, F., Chapot, D. and Thomas, D. (1975), FEBS Lett. 59,263–267

    Google Scholar 

  • Darnoko, D., Cheryan, M. and Artz, W. E. (1989), Enzyme Microb. Technol. 11, 154–159.

    Google Scholar 

  • Garton, L., Appelguist, R., Johansson, G., Scheller, F. and Kirstein, D. (1989), J. Chem. Technol. Biotechnol. 46, 327–333.

    Google Scholar 

  • Graber, M. and Combes, D.(1990), Biotechnol.. Bioeng. 36,12.

    Google Scholar 

  • Houng, J.-Y., Chiou, J.-Y. and Chen, K.-C. (1992), Bioprocess Eng. 8, 85–90.

    Google Scholar 

  • Kawakami, M., Koya, H. and Gond, S. (1992), Fukuoka Kogyo Daigaku Erekutoronikusu Kenkyusho. 9, 65–69.

    Google Scholar 

  • Pfeiffer, D., Scheller, F., Janchen, M., Bertermann, K. and Weise, H. (1980), Anal. Lett. 13, 1179–1200.

    Google Scholar 

  • Qu, H.-B., Zhang, X.-E. and Zhang, S.-Z.(1994), Food Chem, 52(2), 187–192.

    Google Scholar 

  • Varadit, M., Adanyi, N., Nagy, G. and Rezessy-Szabo, J.(1993), Biosensors & Bioelectronics, 8, 339–345.

    Google Scholar 

  • Zhou, L. Q. and Cass, A. E. G.(1991), Biosensors &. Bioelectronics, 6, 445–450.

    Google Scholar 

Download references

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Author notes

  1. Hong-Bo Qu

    Present address: National Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, 100080, Beijing, P. R. China

  2. Wen-Wu Chui

    Present address: Chinese Medical College of Hubei, 430070, Wuhan, P.R. China

Authors and Affiliations

  1. Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, P.R. China

    Hong-Bo Qu, Xian-En Zhang & Wen-Wu Chui

  2. Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing, P. R. China

    Shu-Zheng Zhang & Gao-Xiang Li

  3. National Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, 100080, Beijing, P. R. China

    Fan Ouyang

Authors
  1. Hong-Bo Qu
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  2. Xian-En Zhang
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  3. Wen-Wu Chui
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  4. Shu-Zheng Zhang
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  5. Gao-Xiang Li
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  6. Fan Ouyang
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Qu, HB., Zhang, XE., Chui, WW. et al. Kinetic study on starch hydrolysis using a glucose/maltose sensing system. Biotechnol Tech 9, 445–450 (1995). https://doi.org/10.1007/BF00160834

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  • DOI: https://doi.org/10.1007/BF00160834

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