Summary
An improved HPLC method is described to separate isopenicillin N from its penicilloic acid and from the reduced and oxidized forms of δ -(L-α-amino-adipyl)-L-cysteinyl-D-valine and of DTT. When applied to assay the activity of isopenicillin N synthase only isopenicillin N and its penicilloic acid could be detected.
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Meesschaert, B.D., Cortés, J., Alvarez, E. et al. An improved HPLC assay of the substrate and products of the isopenicillin N synthase. Biotechnol Tech 1, 137–140 (1987). https://doi.org/10.1007/BF00159337
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DOI: https://doi.org/10.1007/BF00159337