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Colormetric detection of horseradish peroxidase labelled DNA using a new chromogen system

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Biotechnology Techniques

Summary

A new chromogen system is described for the detection of horseradish peroxidase (E.C. 1.11.1.7) labelled DNA. Lambda DNA was used as a model and the new system compared favourably with an existing method. The results show the technique should be suitable for single copy gene detection with a lower detection limit of 1–5pg.

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Authors and Affiliations

  1. The Biotechnology Centre, Cranfield Institute of Technology, Cranfield, MK43 OAL, Bedford, U.K.

    Mark E. A. Downs, Philip J. Warner & Anthony P. F. Turner

Authors
  1. Mark E. A. Downs
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  2. Philip J. Warner
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  3. Anthony P. F. Turner
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Downs, M.E.A., Warner, P.J. & Turner, A.P.F. Colormetric detection of horseradish peroxidase labelled DNA using a new chromogen system. Biotechnol Tech 1, 129–134 (1987). https://doi.org/10.1007/BF00159335

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  • DOI: https://doi.org/10.1007/BF00159335

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