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Screening method for microorganisms producing L-aminoacids from D,L-5-monosubstituted hydantoins

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Biotechnology Techniques

Summary

The ability of microorganisms to produce hydantoinase and L-N-carbamoylase could be established by an overlay assay. Enzyme producing strains form clear areas around their colonies caused by the cleavage of D,L-indolylmethylhydantoin. A second overlayer with a tryptophan-auxotroph yeast enables us to detect microorganisms which are able to produce L-tryptophan from D,L-indolylmethylhydantoin.

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Authors and Affiliations

  1. Institute of Biochemistry and Biotechnology, Technical University of Braunschweig, Bültenweg 17, D-3300, Braunschweig, F.R.G.

    C. Groβ, C. Syldatk & F. Wagner

Authors
  1. C. Groβ
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  2. C. Syldatk
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  3. F. Wagner
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Groβ, C., Syldatk, C. & Wagner, F. Screening method for microorganisms producing L-aminoacids from D,L-5-monosubstituted hydantoins. Biotechnol Tech 1, 85–90 (1987). https://doi.org/10.1007/BF00159327

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  • DOI: https://doi.org/10.1007/BF00159327

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